Construct: ORF TRCN0000471208
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010005.1_s317c1
- Derived from:
- ccsbBroadEn_14340
- DNA Barcode:
- TTATACCTCTCCTCCCATAGCCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALKBH8 (91801)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471208
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91801 | ALKBH8 | alkB homolog 8, tRNA methyl... | XM_017018557.1 | 66.5% | 66.3% | (many diffs) |
| 2 | human | 91801 | ALKBH8 | alkB homolog 8, tRNA methyl... | XM_017018554.2 | 38.7% | 38.5% | 699_769del;781G>C;786_1842del |
| 3 | human | 91801 | ALKBH8 | alkB homolog 8, tRNA methyl... | XM_017018553.1 | 38.5% | 38.4% | (many diffs) |
| 4 | human | 91801 | ALKBH8 | alkB homolog 8, tRNA methyl... | NM_138775.3 | 35.7% | 35.6% | 699_769del;781G>C;786_1992del |
| 5 | human | 91801 | ALKBH8 | alkB homolog 8, tRNA methyl... | NM_001301010.2 | 35.6% | 35.5% | (many diffs) |
| 6 | human | 91801 | ALKBH8 | alkB homolog 8, tRNA methyl... | XR_001748035.1 | 18.8% | (many diffs) | |
| 7 | human | 91801 | ALKBH8 | alkB homolog 8, tRNA methyl... | XM_005271728.2 | 15.1% | 15% | (many diffs) |
| 8 | human | 91801 | ALKBH8 | alkB homolog 8, tRNA methyl... | XM_005271729.3 | 15.1% | 15% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 780
- ORF length:
- 714
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cagcaaccat caaagtaatt acaaactcag taaaactgag aagaagttct 121 taaggaaaca gattaaagcc aagcatactt tgctgagaca tgaaggcatt gagacagtat 181 cctatgccac tcagagcctg gttgttgcca atggtggttt gggtaatggt gtgagtcGGA 241 ACCAGCTGCT CCCGGTTTTA GAGAAATGTG GACTGGTGGA TGCTCTCTTA ATGCCACCTA 301 ACAAGCCGTA CTCATTTGCA AGATACAGAA CTACAGAAGA ATCTAAGAGA GCCTATGTTA 361 CCCTCAATGG AAAAGAAGTA GTGGATGATT TAGGACAAAA GATCACTCTG TATTTGAATT 421 TTGTGGAAAA AGTGCAGTGG AAGGAGTTGA GGCCTCAAGC CTTACCACCA GGACTCATGG 481 TAGTAGAAGA AATAATTTCT TCTGAGGAGG AGAAAATGCT TTTGGAAAGT GTTGATTGGA 541 CAGAAGATAC AGACAATCAA AACTCTCAAA AATCCTTAAA ACACAGAAGA GTAAAGCATT 601 TTGGTTATGA GTTCCACTAT GAGAACAACA ATGTAGATAA AGATAAGCCA TTATCTGGGG 661 GTCTTCCTGA CATTTGTGAA AGCTTTTTGG AGAAATGGTT GAGGAAAGGT TACATTAAAC 721 ATAAACCTGA TCAAATGACC ATAAATCAGT ATGAACCTGG GCAAGATTGT CATGCATTTT 781 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 841 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 901 TTTATATATC TTGTGGAAAG GACGATTATA CCTCTCCTCC CATAGCCAGA CGCGTTAAGT 961 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt