Construct: ORF TRCN0000471226
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014338.1_s317c1
- Derived from:
- ccsbBroadEn_00101
- DNA Barcode:
- GTCTCTCTAATTTTTACGCCATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RHOC (389)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471226
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 389 | RHOC | ras homolog family member C | NM_001042678.1 | 100% | 100% | |
| 2 | human | 389 | RHOC | ras homolog family member C | NM_001042679.1 | 100% | 100% | |
| 3 | human | 389 | RHOC | ras homolog family member C | NM_175744.4 | 100% | 100% | |
| 4 | human | 387 | RHOA | ras homolog family member A | NM_001313941.2 | 77.5% | 91.7% | (many diffs) |
| 5 | human | 387 | RHOA | ras homolog family member A | NM_001664.4 | 77.5% | 91.7% | (many diffs) |
| 6 | human | 387 | RHOA | ras homolog family member A | NM_001313944.2 | 69.1% | 81.3% | (many diffs) |
| 7 | mouse | 11853 | Rhoc | ras homolog family member C | NM_001291859.1 | 92% | 100% | (many diffs) |
| 8 | mouse | 11853 | Rhoc | ras homolog family member C | NM_007484.2 | 92% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 645
- ORF length:
- 579
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcaatccga aagaagctgg tgatcgttgg ggatggtgcc tgtgggaaga 121 cctgcctcct catcgtcttc agcaaggatc agtttccgga ggtcTACGTC CCTACTGTCT 181 TTGAGAACTA TATTGCGGAC ATTGAGGTGG ACGGCAAGCA GGTGGAGCTG GCTCTGTGGG 241 ACACAGCAGG GCAGGAAGAC TATGATCGAC TGCGGCCTCT CTCCTACCCG GACACTGATG 301 TCATCCTCAT GTGCTTCTCC ATCGACAGCC CTGACAGCCT GGAAAACATT CCTGAGAAGT 361 GGACCCCAGA GGTGAAGCAC TTCTGCCCCA ACGTGCCCAT CATCCTGGTG GGGAATAAGA 421 AGGACCTGAG GCAAGACGAG CACACCAGGA GAGAGCTGGC CAAGATGAAG CAGGAGCCCG 481 TTCGGTCTGA GGAAGGCCGG GACATGGCGA ACCGGATCAG TGCCTTTGGC TACCTTGAGT 541 GCTCAGCCAA GACCAAGGAG GGAGTGCGGG AGGTGTTTGA GATGGCCACT CGGGCTGGCC 601 TCCAGGTCCG CAAGAACAAG CGTCGGAGGG GCTGTCCCAT TCTCTGCCCA ACTTTCTTGT 661 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 721 AGTAATGAAC TTTATATATC TTGTGGAAAG GACGAGTCTC TCTAATTTTT ACGCCATTTA 781 CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt