Construct: ORF TRCN0000471253
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008699.1_s317c1
- Derived from:
- ccsbBroadEn_05042
- DNA Barcode:
- AACCCTATTTCCATTGACTATCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDPF1 (150383)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471253
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 150383 | CDPF1 | cysteine rich DPF motif dom... | NM_207327.5 | 100% | 100% | |
| 2 | human | 150383 | CDPF1 | cysteine rich DPF motif dom... | XM_011529965.2 | 100% | 100% | |
| 3 | human | 150383 | CDPF1 | cysteine rich DPF motif dom... | XM_011529963.2 | 70.2% | 38.4% | (many diffs) |
| 4 | human | 150383 | CDPF1 | cysteine rich DPF motif dom... | XM_011529964.2 | 67.4% | 33.9% | (many diffs) |
| 5 | human | 150383 | CDPF1 | cysteine rich DPF motif dom... | XM_011529960.2 | 56.6% | 39.9% | 225_228delGGTG;374_651del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 459
- ORF length:
- 369
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCGTCCCA TGTAGAGTGC CGTCCTCTGG 121 GAGTGTTTGA GTGTGAACTC TGTACCTTGA CAGCTCCGTA CAGCTATGTG GGACAGAAGC 181 CCCCCAACAC CCAGTCGATG GTCCTCCTGG AGGAAAGCTA TGTCATGAAG GATCCCTTCA 241 CCTCCGACAA GGACAGATTC CTGGTCCTCG GCTCGTGCTG CAGTTTGTGC AGCAGGCTGG 301 TGTGTGTGGG CCCGGAATGC AGTTTATTCT ACTCCAAGAG ATTCTGCCTC CCTTGTGTCC 361 GGGAGAACAT CAATGCTTTT CCTCAGGAAA TTCGGCAAGA CTTGGAGAAA AGGAAAGCTC 421 CATCAAAGAG GACCCCCAGC CAGCCCGGTT CTCGGACGTG CCCAACTTTC TTGTACAAAG 481 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 541 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 601 ACGAAACCCT ATTTCCATTG ACTATCTCAC GCGTTAAGTC gacaatcaac ctctggatta 661 caaaatttgt gaaagatt