Construct: ORF TRCN0000471259
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008589.1_s317c1
- Derived from:
- ccsbBroadEn_14539
- DNA Barcode:
- TCTCAGAAGGAACAAGGCCCACCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALDH1A3 (220)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471259
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 220 | ALDH1A3 | aldehyde dehydrogenase 1 fa... | NM_001293815.2 | 13.6% | 10.2% | (many diffs) |
| 2 | human | 220 | ALDH1A3 | aldehyde dehydrogenase 1 fa... | NM_000693.4 | 10.7% | 8.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 194
- ORF end:
- 377
- ORF length:
- 183
- Sequence:
-
1 CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG 61 GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA 121 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGCTAGCATC GATTGATCAA CAAGTTTGTA 181 CAAAAAAGTT GGCATGGCCA CCGCTAACGG GGCCGTGGAA AACGGGCAGC CGGACAGGAA 241 GCCGCCGGCC CTGCCGCGCC CCATCCGCAA CCTGGAGGTC AAGTTCACCA AGGTGAGGCG 301 GGCGCCCCTC CCACCCGACG GCCGCGGGCC CCTGCGCTGG GCAGCCAGAC TCGGGGCGGC 361 GGGACTCGGC GCTGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 421 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 481 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TCTCAGAAGG AACAAGGCCC 541 ACCGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt