Construct: ORF TRCN0000471263
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010245.1_s317c1
- Derived from:
- ccsbBroadEn_00349
- DNA Barcode:
- AAACACACACGCACATCCCTCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COX4I1 (1327)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471263
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1327 | COX4I1 | cytochrome c oxidase subuni... | NM_001318786.2 | 99.8% | 100% | 184T>C |
| 2 | human | 1327 | COX4I1 | cytochrome c oxidase subuni... | NM_001861.6 | 99.8% | 100% | 184T>C |
| 3 | human | 1327 | COX4I1 | cytochrome c oxidase subuni... | XM_024450156.1 | 96.3% | 96.5% | 1_18del;202T>C |
| 4 | human | 1327 | COX4I1 | cytochrome c oxidase subuni... | NM_001318788.2 | 82.6% | 72.4% | 184T>C;372_373ins22;420_421ins65 |
| 5 | human | 1327 | COX4I1 | cytochrome c oxidase subuni... | NM_001318794.2 | 79.4% | 72% | (many diffs) |
| 6 | human | 1327 | COX4I1 | cytochrome c oxidase subuni... | NM_001318797.2 | 77.3% | 77.5% | 0_1ins114;70T>C |
| 7 | human | 1327 | COX4I1 | cytochrome c oxidase subuni... | NM_001318802.2 | 35.3% | 29.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 573
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ggctaccagg gtatttagcc tagttggcaa gcgagcaatt tccacctctg 121 tgtgtgtacg agcTCATGAA AGTGTTGTGA AGAGCGAAGA CTTTTCGCTC CCAGCTTATA 181 TGGATCGGCG TGACCACCCC TTGCCGGAGG TGGCCCATGT CAAGCACCTG TCTGCCAGCC 241 AGAAGGCACT GAAGGAGAAG GAGAAGGCCT CCTGGAGCAG CCTCTCCATG GATGAGAAAG 301 TCGAGTTGTA TCGCATTAAG TTCAAGGAGA GCTTTGCTGA GATGAACAGG GGCTCGAACG 361 AGTGGAAGAC GGTTGTGGGC GGTGCCATGT TCTTCATCGG TTTCACCGCG CTCGTTATCA 421 TGTGGCAGAA GCACTATGTG TACGGCCCCC TCCCGCAAAG CTTTGACAAA GAGTGGGTGG 481 CCAAGCAGAC CAAGAGGATG CTGGACATGA AGGTGAACCC CATCCAGGGC TTAGCCTCCA 541 AGTGGGACTA CGAAAAGAAC GAGTGGAAGA AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 601 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 661 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 721 ACACACACGC ACATCCCTCC TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 781 ttgtgaaaga tt