Construct: ORF TRCN0000471282
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005552.1_s317c1
- Derived from:
- ccsbBroadEn_00968
- DNA Barcode:
- AAGCCCGACTCCATTGTAATTATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAGEA9 (4108)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471282
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 728269 | MAGEA9B | MAGE family member A9B | NM_001080790.1 | 100% | 100% | |
| 2 | human | 4108 | MAGEA9 | MAGE family member A9 | NM_005365.4 | 100% | 100% | |
| 3 | human | 4108 | MAGEA9 | MAGE family member A9 | XM_005262334.3 | 100% | 100% | |
| 4 | human | 4108 | MAGEA9 | MAGE family member A9 | XM_005262335.3 | 100% | 100% | |
| 5 | human | 728269 | MAGEA9B | MAGE family member A9B | XM_005278192.3 | 100% | 100% | |
| 6 | human | 728269 | MAGEA9B | MAGE family member A9B | XM_005278193.3 | 100% | 100% | |
| 7 | human | 4108 | MAGEA9 | MAGE family member A9 | XM_024452381.1 | 100% | 100% | |
| 8 | human | 4108 | MAGEA9 | MAGE family member A9 | XM_024452382.1 | 100% | 100% | |
| 9 | human | 728269 | MAGEA9B | MAGE family member A9B | XM_024452436.1 | 100% | 100% | |
| 10 | human | 728269 | MAGEA9B | MAGE family member A9B | XM_024452437.1 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1011
- ORF length:
- 945
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tctcgagcag aggagtccgc actgcaagcc tgatgaagac cttgaagccc 121 aaggagagga cttgggcctg atgggtgcac aggaacccac aggcgaggag gaggagacta 181 cctcctcctc tgacagcaag gaggaggagg tgtctgctgc tgggtcatca agtcctcccc 241 agagtcctca gggaggcgct tcctcctcca tttccgtcta ctacacttta tggagccaat 301 tcgatgaggg ctccagcagt caagaagagg aagagccaag ctcctcggtc gacccagctc 361 agctggagtt catgttccaa gaagcactga aattgaaggt ggctgagttg gttcatttcc 421 tgctccacaa atatcgagtc aaggagccgg tcacaaaggc agaaatgctg gagagcgtca 481 tcaaaaatta caagcgctac tttcctgtga tcttcggcaa agcctccgag ttcatgcagg 541 tgatctttgg cactgatgtg aaggaggtgg accccgccgg ccactcctac aTCCTTGTCA 601 CTGCTCTTGG CCTCTCGTGC GATAGCATGC TGGGTGATGG TCATAGCATG CCCAAGGCCG 661 CCCTCCTGAT CATTGTCCTG GGTGTGATCC TAACCAAAGA CAACTGCGCC CCTGAAGAGG 721 TTATCTGGGA AGCGTTGAGT GTGATGGGGG TGTATGTTGG GAAGGAGCAC ATGTTCTACG 781 GGGAGCCCAG GAAGCTGCTC ACCCAAGATT GGGTGCAGGA AAACTACCTG GAGTACCGGC 841 AGGTGCCCGG CAGTGATCCT GCGCACTACG AGTTCCTGTG GGGTTCCAAG GCCCACGCTG 901 AAACCAGCTA TGAGAAGGTC ATAAATTATT TGGTCATGCT CAATGCAAGA GAGCCCATCT 961 GCTACCCATC CCTTTATGAA GAGGTTTTGG GAGAGGAGCA AGAGGGAGTC TGCCCAACTT 1021 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1081 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1141 CTTGTGGAAA GGACGAAAGC CCGACTCCAT TGTAATTATA ACGCGTTAAG TCgacaatca 1201 acctctggat tacaaaattt gtgaaagatt