Construct: ORF TRCN0000471285
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007384.1_s317c1
- Derived from:
- ccsbBroadEn_11069
- DNA Barcode:
- TCCTGTTCCCTAGCCTTGCCGCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTGER3 (5733)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471285
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_198718.2 | 89.9% | 87.7% | (many diffs) |
| 2 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | XM_011541810.3 | 89.5% | 86.9% | (many diffs) |
| 3 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_198715.3 | 87.6% | 84.5% | (many diffs) |
| 4 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_001126044.2 | 87.3% | 86% | (many diffs) |
| 5 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_198714.2 | 87.3% | 86% | (many diffs) |
| 6 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_198719.2 | 87.3% | 86% | (many diffs) |
| 7 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_198716.2 | 86.5% | 85.8% | (many diffs) |
| 8 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NM_198717.2 | 84.7% | 84.3% | (many diffs) |
| 9 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | XR_946714.2 | 76% | (many diffs) | |
| 10 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NR_028292.2 | 60.2% | (many diffs) | |
| 11 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NR_028293.2 | 60% | (many diffs) | |
| 12 | human | 5733 | PTGER3 | prostaglandin E receptor 3 | NR_028294.2 | 58.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1356
- ORF length:
- 1287
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaggagacc cggggctacg gaggggatgc ccccttctgc acccgcctca 121 accactccta cacaggcatg tgggcgcccg agcgttccgc cgaggcgcgg ggcaacctca 181 cgcgccctcc agggtctggc gaggattgcg gatcggtgtc cgtggccttc ccgatcacca 241 tgctgctcac tggtttcgtg ggcaacgcac tggccatgct gctcgtgtcg cgcagctacc 301 ggcgccggga gagcaagcgc aagaagtcct tcctgctgtg catcggctgg ctggcgctca 361 ccgacctggt cgggcagctt ctcaccaccc cggtcgtcat cgtcgtgtac ctgtccaagc 421 agcgttggga gcacatcgac ccgtcggggc ggctctgcac ctttttcggg ctgaccatga 481 ctgttttcgg gctctcctcg ttgttcatcg ccagcgccat ggccgtcgag cgggcgctgg 541 ccatcagggc gccgcactgg tatgcgagcc acatgaagac gcgtgccacc cgcgctgtgc 601 tgctcggcgt gtggctggcc gtgctcgcct tcgccctgct gccggtgctg ggcgtgggcc 661 agtacaccgt ccagtggccc gggacgtggt gcttcatcag caccgggcga gggggcaacg 721 ggactagctc ttcgcataac tggggcaacc ttttcttcgc ctctgccttt gccttcctgg 781 ggctcttggc gctgacagtc accttttcct gcaacctggc caccattaag gccctggtgt 841 cccgctgccg ggccaaggcc acggcatctc agtccagtgc ccagtggggc cgcatcacga 901 ccgagacggc cattcagctt atgggGATCA TGTGCGTGCT GTCGGTCTGC TGGTCTCCGC 961 TCCTGATAAT GATGTTGAAA ATGATCTTCA ATCAGACATC AGTTGAGCAC TGCAAGACAC 1021 ACACGGAGAA GCAGAAAGAA TGCAACTTCT TCTTAATAGC TGTTCGCCTG GCTTCACTGA 1081 ACCAGATCTT GGATCCTTGG GTTTACCTGC TGTTAAGAAA GATCCTTCTT CGAAAGTTTT 1141 GCCAGATGAG AAAAAGAAGA CTCAGAGAGC AATTCTGGAG GCCGGCAAGT TCAGGATCAG 1201 GGTGCCAGCA GATTCGGTGT CTGATGGAGT CTTGCTCTCT CACCCAGACT GGAGTGCAGT 1261 GGAGTGATTT CCGCTCACTG CAACCTTCAC CTCCTCCACT CACTGCAATC TTCGCCTCCT 1321 GGGTTCAAGT GATTCTCCTG CCTCAGCCTC CCAAGTTGCC AACTTTCTTG TACAAAGTGG 1381 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1441 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1501 ATCCTGTTCC CTAGCCTTGC CGCAAACGCG TTAAGTCgac aatcaacctc tggattacaa 1561 aatttgtgaa agatt