Construct: ORF TRCN0000471292
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015168.1_s317c1
- Derived from:
- ccsbBroadEn_03527
- DNA Barcode:
- TTGCGAAGCCCGGTTCGCTTTGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC6A15 (55117)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471292
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55117 | SLC6A15 | solute carrier family 6 mem... | NM_182767.6 | 100% | 100% | |
| 2 | human | 55117 | SLC6A15 | solute carrier family 6 mem... | NM_001146335.3 | 85.3% | 85.3% | 0_1ins321 |
| 3 | human | 55117 | SLC6A15 | solute carrier family 6 mem... | XM_011538525.3 | 71.9% | 68.8% | (many diffs) |
| 4 | human | 55117 | SLC6A15 | solute carrier family 6 mem... | NM_018057.6 | 37.3% | 35.4% | (many diffs) |
| 5 | mouse | 103098 | Slc6a15 | solute carrier family 6 (ne... | NM_001252330.1 | 86.7% | 91.5% | (many diffs) |
| 6 | mouse | 103098 | Slc6a15 | solute carrier family 6 (ne... | NM_175328.3 | 86.7% | 91.5% | (many diffs) |
| 7 | mouse | 103098 | Slc6a15 | solute carrier family 6 (ne... | XM_006512982.3 | 86.7% | 91.5% | (many diffs) |
| 8 | mouse | 103098 | Slc6a15 | solute carrier family 6 (ne... | XM_006512983.3 | 86.7% | 91.5% | (many diffs) |
| 9 | mouse | 103098 | Slc6a15 | solute carrier family 6 (ne... | XM_006512984.3 | 86.7% | 91.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2256
- ORF length:
- 2190
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc caaaaatagc aaggtggtaa aaagagaatt agatgatgat gttactgagt 121 ctgtcaaaga ccttctttcc aatgaagacg cagctgatga tgcttttaag acaagtgaac 181 taattgttga tggccaggaa gagaaagata cagatgttga agaaggatct gaagtcgaag 241 atgaaagacc agcttggaac agtaaactac aatacatcct ggcccaagtt ggattttctg 301 taggtttagg aaatgtgtgg cgatttccat acctatgtca gaagaatggg ggcggtgcat 361 atcttttacc atatttaata ctacttatgg taataggtat tccccttttt ttcttggaac 421 tctctgtggg tcaaagaatt cggcgaggca gcattggtgt atggaattac ataagcccta 481 aactgggcgg gattggattt gcaagttgtg tagtgtgcta ttttgtagct ctctactaca 541 acgtcatcat tggctggagt ttgttttatt tttctcagtc ttttcagcaa cccctgcctt 601 gggatcagtg tcctttggtg aaaaatgctt cacacacttt tgtagaacca gaatgtgaac 661 aaagttctgc caccacctat tactggtaca gggaagcact gaatatttca agttccattt 721 ctgaaagtgg gggcttaaac tggaagatga ccatctgctt gttggctgcc tgggtcatgg 781 tttgcttggc tatgatcaaa ggcattcagt cttctggaaa aatcatatat tttagttctc 841 tgtttccata tgtggtactt atttgcttcc tcatcagagc attcctttta aatggttcaa 901 ttgatggcat tcgccacatg tttaccccta agcttgaaat aatgctggag cccaaggtct 961 ggagagaagc tgctactcaa gtgttctttg ccttaggtct gggatttggt ggtgtcattg 1021 ccttttcaag ctacaacaag agagacaaca actgccactt tgatgctgtc ctggtgtcct 1081 tcatcaattt tttcacttct gtcctggcaa cattggtggt gtttgcagtt ctgggcttca 1141 aagcaaatgt cataaatgag aaatgcatta cacaaaattc agagacgatc atgaaatttt 1201 tgaaaatggg gaacattagt caggatatta ttccccatca tatcaacctt tcaactgtta 1261 ctgcagaaga ttatcattta gtttatgaca tcattcaaaa agtgaaagaa gaagagtttc 1321 ctgctcttca tctcaattcc tgtaaaattg aagaagagct aaataaagct gttcagggga 1381 ccggcttagc ttttattgcc tttacagaag cgatgacaca ttttcctgca tctcccttct 1441 ggtcagtgat gtttttcctc atgctggtca atctaggcct tggcagtatg tttggaacca 1501 ttgaagggat tgtcacgcct attgtggaca ctttcaaagt gaggaaagaa attcttactg 1561 ttatctgttg tcttctggca ttttgtattg gcctgatatt tgtgcaacgc tctggaaatt 1621 actttgttac aatgtttgat gattattctg ctacactgcc tctgctaatt gtagtcattt 1681 tggagaatat tgctgtatgc tttgtttatg gcatagataa gtttatggaa gacctaaaag 1741 atatgctggg ctttgctccc agcagatatt actactatat gtggaaatat atttctcctc 1801 taatgctatt atcattgcta atagctagtg ttgtgaatat gggattaagT CCTCCTGGCT 1861 ATAACGCATG GATTGAAGAT AAGGCATCTG AAGAATTTCT GAGCTATCCA ACATGGGGAC 1921 TGGTTGTTTG TGTCTCTCTG GTTGTCTTTG CAATACTCCC AGTCCCTGTA GTTTTCATTG 1981 TTCGTCGCTT CAACCTTATA GATGATAGTT CTGGTAATTT AGCATCTGTG ACCTATAAGA 2041 GAGGAAGGGT CCTGAAAGAG CCTGTGAACT TAGAGGGCGA TGATACAAGC CTCATTCACG 2101 GAAAAATACC GAGCGAGATG CCATCTCCAA ATTTTGGTAA AAATATTTAT CGAAAACAGA 2161 GTGGATCCCC AACTCTGGAT ACTGCTCCCA ATGGACGGTA TGGAATAGGG TACTTGATGG 2221 CAGATATTAT GCCAGATATG CCAGAATCTG ATTTGTACCC AACTTTCTTG TACAAAGTGG 2281 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2341 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2401 ATTGCGAAGC CCGGTTCGCT TTGCCACGCG TTAAGTCgac aatcaacctc tggattacaa 2461 aatttgtgaa agatt