Construct: ORF TRCN0000471314
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003202.1_s317c1
- Derived from:
- ccsbBroadEn_15996
- DNA Barcode:
- CTTGTGTTGATGCCGATAGGTAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DHDDS (79947)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471314
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79947 | DHDDS | dehydrodolichyl diphosphate... | NM_001243565.1 | 100% | 100% | |
| 2 | human | 79947 | DHDDS | dehydrodolichyl diphosphate... | NM_001243564.1 | 90.4% | 89.2% | (many diffs) |
| 3 | human | 79947 | DHDDS | dehydrodolichyl diphosphate... | NM_205861.3 | 88.2% | 88.2% | 322_438del |
| 4 | human | 79947 | DHDDS | dehydrodolichyl diphosphate... | NM_024887.3 | 88% | 88% | 322_438del;763_765delCAG |
| 5 | human | 79947 | DHDDS | dehydrodolichyl diphosphate... | NM_001319959.1 | 74.5% | 60.3% | (many diffs) |
| 6 | mouse | 67422 | Dhdds | dehydrodolichyl diphosphate... | NM_026144.4 | 78.4% | 81.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 948
- ORF length:
- 882
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc atggatcaag gaaggagagc tgtcactttg ggagcggttc tgtgccaaca 121 tcataaaggc aggcccaatg ccgaaacaca ttgcattcat aatggacggg aaccgtcgct 181 atgccaagaa gtgccaggtg gagcggcagg aaggccactc acagggcttc aacaagctag 241 ctgagactct gcggtggtgt ttgaacctgg gcatcctaga ggtgacagtc tacgcattca 301 gcattgagaa cttcaaacgc tccaagagtg aggtagacgg gcttatggat ctggcccggc 361 agaagttcag ccgcttgatg gaagaaaagt gtttcctgaa tgtctgtttt gcatacacat 421 cccgtcatga gatcagcaat gctgtgagag agatggcctg gggggtggag caaggcctgt 481 tggatcccag tgatatctct gagtctctgc ttgataagtg cctctatacc aaccgctctc 541 ctcatcctga catcttgata cggacttctG GAGAAGTGCG GCTGAGTGAC TTCTTGCTAT 601 GGCAGACCTC TCACTCCTGC CTGGTGTTCC AACCCGTTCT GTGGCCAGAG TATACATTTT 661 GGAACCTCTT CGAGGCCATC CTGCAGTTCC AGATGAACCA TAGCGTGCTT CAGAAGGCCC 721 GAGACATGTA TGCAGAGGAG CGGAAGAGGC AGCAGCTGGA GAGGGACCAG GCTACAGTGA 781 CAGAGCAGCT GCTGCGAGAG GGGCTCCAAG CCAGTGGGGA CGCCCAGCTC CGAAGGACAC 841 GCTTGCACAA ACTCTCGGCC AGACGGGAAG AGCGAGTCCA AGGCTTCCTG CAGGCCTTGG 901 AACTCAAGCG AGCTGACTGG CTGGCCCGTC TGGGCACTGC ATCAGCCTGC CCAACTTTCT 961 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1021 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1081 GTGGAAAGGA CGACTTGTGT TGATGCCGAT AGGTAGCACG CGTTAAGTCg acaatcaacc 1141 tctggattac aaaatttgtg aaagatt