Construct: ORF TRCN0000471333
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015369.1_s317c1
- Derived from:
- ccsbBroadEn_08241
- DNA Barcode:
- TTGCCTCAACAACTTCTACTGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HAO2 (51179)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471333
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51179 | HAO2 | hydroxyacid oxidase 2 | NM_016527.4 | 99.9% | 100% | 16T>C |
| 2 | human | 51179 | HAO2 | hydroxyacid oxidase 2 | NM_001005783.3 | 96.3% | 96.4% | 1_39del;55T>C |
| 3 | human | 51179 | HAO2 | hydroxyacid oxidase 2 | XM_024447493.1 | 77.1% | 77.1% | 1_39del;55T>C;600_601ins210 |
| 4 | human | 51179 | HAO2 | hydroxyacid oxidase 2 | XM_024447485.1 | 76% | 75.8% | 16T>C;928_929ins70;984_1221del |
| 5 | human | 51179 | HAO2 | hydroxyacid oxidase 2 | XM_017001421.1 | 73.8% | 73.6% | (many diffs) |
| 6 | human | 51179 | HAO2 | hydroxyacid oxidase 2 | XM_024447484.1 | 73.8% | 73.6% | (many diffs) |
| 7 | human | 51179 | HAO2 | hydroxyacid oxidase 2 | XM_011541558.2 | 70.2% | 69.9% | (many diffs) |
| 8 | human | 51179 | HAO2 | hydroxyacid oxidase 2 | XM_024447495.1 | 70.2% | 70.3% | (many diffs) |
| 9 | human | 51179 | HAO2 | hydroxyacid oxidase 2 | XM_024447486.1 | 68.1% | 67.8% | (many diffs) |
| 10 | human | 51179 | HAO2 | hydroxyacid oxidase 2 | XM_024447492.1 | 58% | 57.4% | (many diffs) |
| 11 | human | 51179 | HAO2 | hydroxyacid oxidase 2 | XM_011541561.2 | 53.3% | 52.1% | (many diffs) |
| 12 | human | 51179 | HAO2 | hydroxyacid oxidase 2 | XM_024447498.1 | 46.4% | 45.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1119
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cttggtgtgt ctgacagact ttcaggccca tgcgcgagag cagctgtcta 121 agtcaactcg ggattttatt gaaggtggag cagatgacag catcacgcgg gatgacaaca 181 ttgcagcatt taaaagaatt cgcctccgtc cgcggtacct gagagatgtg tctgaggtgg 241 acaccagaac cacaatccaa ggggaggaga tcagtgcccc tatttgtatc gcacccacag 301 ggttccactg ccttgtctgg cctgatgggg aaatgagcac agcaagagct gcccaagcgg 361 ctggtatctg ctacatcacc agcacatttg ccagctgtag ccttgaagac attgtcattg 421 cagctcccga aggcctccga tggttccaac tctatgtgca tccagacctg cagctgaaca 481 aacagttgat ccagagggta gaatccctag gtttcaaagc tttggtaata actttggata 541 cacctgtatg tggcaacagg cgacatgaca ttcgaaacca gttgaggagg aacttaacac 601 taacagatct tcaatcacct aaaaagggaa atgcaatacc ttatttccag atgactccta 661 tcagcacttc tctctgctgg aatgatctct cctggtttca gagcataact cgattgccca 721 tcatccTGAA AGGGATTTTG ACAAAAGAGG ATGCAGAGTT AGCTGTGAAG CACAATGTCC 781 AGGGTATCAT TGTTTCCAAC CATGGTGGGA GGCAGCTTGA TGAGGTTCTT GCTTCAATTG 841 ATGCTTTGAC AGAAGTGGTG GCTGCTGTAA AGGGGAAAAT TGAAGTCTAC CTGGATGGCG 901 GGGTCCGAAC TGGCAATGAT GTGCTGAAGG CTCTGGCCCT TGGAGCTAAG TGCATTTTTC 961 TTGGGAGACC AATCCTATGG GGCCTTGCCT GCAAGGGTGA ACATGGTGTT AAGGAAGTTT 1021 TGAACATTTT AACAAATGAG TTCCACACTT CCATGGCCCT TACAGGCTGC CGGTCGGTCG 1081 CTGAGATCAA TCGAAACTTG GTCCAGTTTT CCAGGCTGTA CCCAACTTTC TTGTACAAAG 1141 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1201 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1261 ACGATTGCCT CAACAACTTC TACTGGGAAC GCGTTAAGTC gacaatcaac ctctggatta 1321 caaaatttgt gaaagatt