Construct: ORF TRCN0000471342
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017011.1_s317c1
- Derived from:
- ccsbBroadEn_08183
- DNA Barcode:
- ATTCTCAGAACAATTGCCATCCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NSDHL (50814)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471342
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50814 | NSDHL | NAD(P) dependent steroid de... | NM_001129765.1 | 99.9% | 100% | 132T>G |
| 2 | human | 50814 | NSDHL | NAD(P) dependent steroid de... | NM_015922.3 | 99.9% | 100% | 132T>G |
| 3 | human | 50814 | NSDHL | NAD(P) dependent steroid de... | XM_011531178.2 | 99.9% | 100% | 132T>G |
| 4 | human | 50814 | NSDHL | NAD(P) dependent steroid de... | XM_017029564.1 | 95.8% | 95.8% | 1_48del;180T>G |
| 5 | mouse | 18194 | Nsdhl | NAD(P) dependent steroid de... | NM_010941.3 | 82.2% | 82.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1185
- ORF length:
- 1119
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga accagcagtt agcgagccaa tgagagacca agtcgcacgg actcatttga 121 cagaggacac tcccaaagtg aatgctgaca tagaaaaggt taaccagaat caggccaaga 181 gatgcacagt gatcgggggc tctggattcc tggggcagca catggtggag cagttgctgg 241 caagaggata tgctgtcaat gtatttgata tccagcaagg gtttgataat ccccaggtgc 301 ggttctttct gggtgacctc tgcagccgac aggatctgta cccagctctg aaaggtgtaa 361 acacagtttt ccactgtgcg tcacccccac catccagtaa caacaaggag ctcttttata 421 gagtgaatta cattggcacc aagaatgtca ttgaaacttg caaagaggct ggggttcaga 481 aactcatttt aaccagcagt gccagtgtca tctttgaggg cgtcgatatc aagaatggaa 541 ctgaagacct tccctatgcc atgaaaccca ttgactacta cacagagact aagatcttac 601 aggagagggc agttctgggc gccaacgatc ctgagaagaa tttcttaacc acagccatcc 661 gccctcatgg cattttcggc ccaagggacc cgcagttggt acccatccTC ATCGAGGCAG 721 CCAGGAACGG CAAGATGAAG TTCGTGATTG GAAATGGGAA GAACTTGGTG GACTTCACCT 781 TTGTGGAGAA CGTGGTCCAT GGACACATCC TGGCGGCAGA GCAGCTCTCC CGAGACTCGA 841 CACTGGGTGG GAAGGCATTT CACATCACCA ATGATGAGCC CATCCCTTTC TGGACATTCC 901 TGTCTCGCAT CCTGACAGGC CTCAATTATG AGGCCCCCAA GTACCACATC CCCTACTGGG 961 TGGCCTACTA CCTGGCCCTC CTGCTATCCC TGCTGGTGAT GGTGATCAGT CCTGTCATCC 1021 AGCTGCAGCC CACCTTCACA CCCATGCGGG TCGCACTGGC TGGCACATTC CACTACTACA 1081 GCTGCGAGAG AGCCAAAAAG GCCATGGGCT ACCAGCCACT AGTGACCATG GATGATGCTA 1141 TGGAGAGGAC CGTGCAGAGC TTTCGCCACC TGCGGAGGGT CAAGTGCCCA ACTTTCTTGT 1201 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1261 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1321 GAAAGGACGA ATTCTCAGAA CAATTGCCAT CCCAACGCGT TAAGTCgaca atcaacctct 1381 ggattacaaa atttgtgaaa gatt