Construct: ORF TRCN0000471347
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003645.1_s317c1
- Derived from:
- ccsbBroadEn_13707
- DNA Barcode:
- GTTAATCGACCCTGTTAGTCCATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHBP19 (494150)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471347
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5245 | PHB | prohibitin | NM_001281496.1 | 61.2% | 57% | (many diffs) |
| 2 | human | 5245 | PHB | prohibitin | NM_001281715.1 | 61.2% | 57% | (many diffs) |
| 3 | human | 5245 | PHB | prohibitin | NM_002634.4 | 61.2% | 57% | (many diffs) |
| 4 | human | 5245 | PHB | prohibitin | XM_017024763.1 | 61.2% | 57% | (many diffs) |
| 5 | human | 5245 | PHB | prohibitin | NM_001281497.1 | 31.9% | 26.1% | (many diffs) |
| 6 | mouse | 18673 | Phb | prohibitin | NM_008831.4 | 57.7% | 56.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 669
- ORF length:
- 603
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg tacagaaacc aattatcttt gcctttctcc accacgtaat gtaccaatca 121 tcactggtag caaagattta cagaatgtca atatcacact gcgcatcaTC TTCCAGCCTG 181 TTGCTAGCCA GCTTCCTCGC ATCTTCACCA GCATCGGAGA GGACTATGAT GAGCCTGTGC 241 TGACGTACAT CACGACCGAG ATCCTCAAGT CAGTGGTGGC TCGCTTTGAT GCTGGAGAAG 301 TTATCACTCA GAGAGAGCTG GTCTCCAGGC AGGTGAGCAA CGACCTTACG GAGCAAGCAG 361 CCACATTTGG GCTCATCCTG GACGACGTGT CCTTGACATA TCTGACCTTT GGAAAGGAGT 421 TCACAGAAGC AGTGGAAGCC AAACAGGTGG CTCAGCAGGA AGCAGAGAGG GCCAGATTTG 481 TGAAGGAAAA GGCTGAGCAG CAGAAAAAGG CTGAGCAGCA GAAAAAGGTT GAGCAGCAGA 541 AAAAGGCAGC CGTGATCTCT GCTGAGGGCG ACTCCAAGGC AACCGAGCTG ATTGTCAACT 601 CACTGGCCAC CGCGGGGGAC GGCCTGATGG AGCTGTGCAA GTTGGAAGCC GCGGAGGCTC 661 TCGGAACATG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 721 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 781 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGTTAAT CGACCCTGTT AGTCCATCAC 841 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt