Construct: ORF TRCN0000471366
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002043.1_s317c1
- Derived from:
- ccsbBroadEn_03878
- DNA Barcode:
- AAACCAAACCGTCATTTATCGTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SENP2 (59343)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471366
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 59343 | SENP2 | SUMO specific peptidase 2 | NM_021627.3 | 100% | 100% | |
| 2 | human | 59343 | SENP2 | SUMO specific peptidase 2 | XM_005247690.3 | 92.5% | 92.5% | 1110_1111ins132 |
| 3 | human | 59343 | SENP2 | SUMO specific peptidase 2 | XM_005247691.3 | 80.4% | 80.4% | 0_1ins345 |
| 4 | human | 59343 | SENP2 | SUMO specific peptidase 2 | XM_017006973.2 | 73% | 73% | 0_1ins345;765_766ins132 |
| 5 | mouse | 75826 | Senp2 | SUMO/sentrin specific pepti... | NM_029457.3 | 88.3% | 87.9% | (many diffs) |
| 6 | mouse | 75826 | Senp2 | SUMO/sentrin specific pepti... | XM_006522688.2 | 87.5% | 87.2% | (many diffs) |
| 7 | mouse | 75826 | Senp2 | SUMO/sentrin specific pepti... | XM_006522689.3 | 81% | 80.3% | (many diffs) |
| 8 | mouse | 75826 | Senp2 | SUMO/sentrin specific pepti... | XM_006522690.1 | 76.4% | 76.2% | (many diffs) |
| 9 | mouse | 75826 | Senp2 | SUMO/sentrin specific pepti... | NR_027488.1 | 34.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1833
- ORF length:
- 1767
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta cagatggctg gttaggattc tcggcaccat tttccgtttc tgcgaccggt 121 cggtgccccc tgcccgggcc ctcctgaaga ggcggcgctc agacagcact ctgttttcta 181 cagtggacac tgatgaaata ccagccaaaa gaccaagatt agattgcttt attcaccaag 241 tgaaaaacag tctctacaat gctgccagct tatttggatt cccattccag ctgaccacaa 301 agcccatggt aacttctgct tgtaatggaa cacggaatgt ggccccttca ggagaggtat 361 tttcgaactc ttcatcttgt gaactgacag gttctggatc ctggaacaac atgctgaaac 421 tgggtaataa atctcctaat ggaataagtg actatccaaa gatcagagtg acagttaccc 481 gagatcagcc acgcagagtc ctgccttcct ttggttttac tttgaactca gaaggctgta 541 atagaagacc aggtggccgt cgccatagca aaggtaatcc agagagttct ttaatgtgga 601 aacctcagga acaggctgta acagagatga tttctgaaga gagtggcaag ggtctgaggc 661 gtccccattg tactgtggag gagggtgttc aaaaagagga aagagagaag taccgaaagt 721 tattggaacg acttaaagaa agtggtcatg gaaactctgt ctgtcctgta acttcaaatt 781 atcacagttc tcaaagaagt cagatggaca cattaaagac caaaggctgg ggggaagagc 841 aaaatcacgg agtcaaaaca actcagtttg ttccaaaaca atatagactt gttgaaacaa 901 ggggacctct atgttcattg agaagtgaaa agaggtgttc aaaggggaaa attactgata 961 cagagacgat ggtcggaatc agatttgaaa atgaaagtag gaggggatac caactggagc 1021 ctgacctatc agaagaagtg tcggcccgac tccgcctggg cagtggaagc aatggcttac 1081 tcaggaggaa agtgtcaata attgagacaa aggaaaagaa ttgctcaggc aaagagaggg 1141 acagaagaac ggacgatctc cttgaactta cagaggacat ggaaaaggaa atcagtaatg 1201 ccctaggcca tggcccacag gatgaaatcc taagtagtgc tttcaaattg cgaattactc 1261 gaggagatat tcagacatta aagaactatc actggctcaa tgatgaagtc attaattttt 1321 acatgaatct tctggtGGAA AGAAATAAAA AGCAAGGCTA TCCAGCACTT CATGTATTCA 1381 GTACTTTCTT CTATCCTAAA TTAAAGTCTG GGGGTTACCA AGCAGTGAAA CGATGGACCA 1441 AAGGGGTAAA TCTCTTTGAA CAAGAAATTA TTCTGGTGCC TATTCATCGG AAGGTACATT 1501 GGAGCCTGGT GGTGATTGAC CTAAGAAAAA AGTGTCTTAA ATATCTGGAT TCTATGGGAC 1561 AAAAGGGCCA CAGGATCTGT GAGATTCTCC TTCAGTATTT ACAGGATGAA AGTAAGACCA 1621 AAAGAAATAG TGATCTGAAT CTTTTAGAGT GGACCCATCA CAGCATGAAA CCACACGAGA 1681 TTCCTCAACA GCTGAATGGG AGTGATTGTG GAATGTTTAC TTGTAAATAT GCAGATTATA 1741 TTTCTAGGGA CAAACCTATC ACATTTACTC AGCACCAGAT GCCTCTCTTC CGGAAGAAGA 1801 TGGTGTGGGA AATCCTTCAT CAGCAGTTGC TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1861 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1921 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 1981 ACCAAACCGT CATTTATCGT ATACGCGTTA AGTCgacaat caacctctgg attacaaaat 2041 ttgtgaaaga tt