Construct: ORF TRCN0000471369

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003060.1_s317c1
Derived from:
ccsbBroadEn_14113
DNA Barcode:
ATATTAAGTTAGTCCCACAAACCT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
TPK1 (27010)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471369
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350885.1 99% 4.3% 14_16delAGAinsG;25A>G
2 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350886.1 99% 4.3% 14_16delAGAinsG;25A>G
3 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350887.1 99% 4.3% 14_16delAGAinsG;25A>G
4 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350889.1 99% 4.3% 14_16delAGAinsG;25A>G
5 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350893.1 99% 4.3% 14_16delAGAinsG;25A>G
6 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350894.1 99% 4.3% 14_16delAGAinsG;25A>G
7 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516047.2 99% 4.3% 14_16delAGAinsG;25A>G
8 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516048.1 99% 4.3% 14_16delAGAinsG;25A>G
9 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011982.1 99% 4.3% 14_16delAGAinsG;25A>G
10 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_024446717.1 99% 4.3% 14_16delAGAinsG;25A>G
11 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350895.1 92.1% 3.1% 0_1ins31;2T>A
12 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_024446716.1 92.1% 3.1% 0_1ins31;2T>A
13 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011980.2 83.2% 3.6% 1_78del;92_94delAGAinsG;103A>G
14 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011981.2 83.2% 3.6% 1_78del;92_94delAGAinsG;103A>G
15 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350882.1 57% 2.5% 1_303del;317_319delAGAinsG;328A>G
16 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350883.1 57% 2.5% 1_303del;317_319delAGAinsG;328A>G
17 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350884.2 57% 2.5% 1_303del;317_319delAGAinsG;328A>G
18 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350879.1 55.8% 2.4% 1_318del;332_334delAGAinsG;343A>G
19 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_022445.4 55.8% 2.4% 1_318del;332_334delAGAinsG;343A>G
20 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_005249970.1 55.8% 2.4% 1_318del;332_334delAGAinsG;343A>G
21 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516039.2 55.8% 2.4% 1_318del;332_334delAGAinsG;343A>G
22 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011974.1 55.8% 2.4% 1_318del;332_334delAGAinsG;343A>G
23 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011975.1 55.8% 2.4% 1_318del;332_334delAGAinsG;343A>G
24 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001042482.1 55.5% 3.6% (many diffs)
25 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350880.1 55.5% 3.6% (many diffs)
26 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516037.2 51.3% 2.2% 1_381del;395_397delAGAinsG;406A>G
27 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011970.1 51.3% 2.2% 1_381del;395_397delAGAinsG;406A>G
28 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011971.1 51.3% 2.2% 1_381del;395_397delAGAinsG;406A>G
29 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516031.1 50.4% 2.2% 1_396del;410_412delAGAinsG;421A>G
30 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516032.2 50.4% 2.2% 1_396del;410_412delAGAinsG;421A>G
31 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516033.2 50.4% 2.2% 1_396del;410_412delAGAinsG;421A>G
32 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516034.2 50.4% 2.2% 1_396del;410_412delAGAinsG;421A>G
33 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350881.1 47.1% 2.4% (many diffs)
34 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516035.3 42.7% 2.2% (many diffs)
35 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011969.1 42.5% 2.2% (many diffs)
36 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011972.1 40.4% 2.4% (many diffs)
37 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516040.2 39.6% 2.4% (many diffs)
38 human 27010 TPK1 thiamin pyrophosphokinase 1 NR_146936.2 14.5% (many diffs)
39 mouse 29807 Tpk1 thiamine pyrophosphokinase XM_006506219.3 53.5% 1.8% (many diffs)
40 mouse 29807 Tpk1 thiamine pyrophosphokinase NM_013861.4 48.2% 1.6% (many diffs)
41 mouse 29807 Tpk1 thiamine pyrophosphokinase XM_006506218.3 32% .8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
90
ORF end:
126
ORF length:
36
Sequence:
1TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT
61GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCTCCAAAA GAGTAGAAGA AGAAGACTTA
121AAGGTTGATG TGATCGTGAC ACTGGGAGGC CTTGCTGGGC GTTTTGACCA GATTATGGCA
181TCTGTGAATA CCTTGTTCCA AGCGACTCAC ATCACTCCTT TTCCAATTAT AATAATCCAA
241GAGGAATCGC TGATCTACCT GCTCCAACCA GGAAAGCACA GGTTGCATGT AGACACTGGA
301ATGGAGGGTG ATTGGTGTGG CCTTATTCCT GTTGGACAGC CTTGTATGCA GGTTACAACC
361ACAGGCCTCA AGTGGAACCT CACAAATGAT GTGCTTGCTT TTGGAACATT GGTCAGTACT
421TCCAATACCT ACGACGGGTC TGGTGTTGTG ACTGTGGAAA CTGACCACCC ACTCCTCTGG
481ACCATGGCCA TCAAAAGCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT
541ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA
601AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATATTA AGTTAGTCCC
661ACAAACCTAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)