Construct: ORF TRCN0000471439
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014901.1_s317c1
- Derived from:
- ccsbBroadEn_08993
- DNA Barcode:
- TGGGAAATCCCAAACACACATTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DHDDS (79947)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471439
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79947 | DHDDS | dehydrodolichyl diphosphate... | NM_205861.3 | 99.7% | 99.6% | 666C>T;757G>A |
| 2 | human | 79947 | DHDDS | dehydrodolichyl diphosphate... | NM_024887.3 | 99.5% | 99.4% | 666C>T;757G>A;763_765delCAG |
| 3 | human | 79947 | DHDDS | dehydrodolichyl diphosphate... | NM_001243564.1 | 89.5% | 89.1% | 440_441ins102;564C>T;655G>A |
| 4 | human | 79947 | DHDDS | dehydrodolichyl diphosphate... | NM_001243565.1 | 88% | 87.9% | 321_322ins117;549C>T;640G>A |
| 5 | human | 79947 | DHDDS | dehydrodolichyl diphosphate... | NM_001319959.1 | 71.8% | 71.7% | 0_1ins279;387C>T;478G>A |
| 6 | mouse | 67422 | Dhdds | dehydrodolichyl diphosphate... | NM_026144.4 | 88.2% | 92.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1065
- ORF length:
- 999
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc atggatcaag gaaggagagc tgtcactttg ggagcggttc tgtgccaaca 121 tcataaaggc aggcccaatg ccgaaacaca ttgcattcat aatggacggg aaccgtcgct 181 atgccaagaa gtgccaggtg gagcggcagg aaggccactc acagggcttc aacaagctag 241 ctgagactct gcggtggtgt ttgaacctgg gcatcctaga ggtgacagtc tacgcattca 301 gcattgagaa cttcaaacgc tccaagagtg aggtagacgg gcttatggat ctggcccggc 361 agaagttcag ccgcttgatg gaagaaaagg agaaactgca gaagcatggg gtgtgtatcc 421 gggtcctggg cgatctgcac ttgttgccct tggatctcca ggagctgatt gcacaagctg 481 tacaggccac gaagaactac aacaagtgtt tcctgaatgt ctgttttgca tacacatccc 541 gtcatgagat cagcaatgct gtgagagaga tggcctgggg ggtggagcaa ggcctgttgg 601 atcccagtga tatctctgag tctctgcttg ataagtgcct ctataccaac cgctctcctc 661 atcctgacat ctTGATACGG ACTTCTGGAG AAGTGCGGCT GAGTGACTTC TTGCTATGGC 721 AGACCTCTCA TTCCTGCCTG GTGTTCCAAC CCGTTCTGTG GCCAGAGTAT ACATTTTGGA 781 ACCTCTTCGA GGCCATCCTG CAGTTCCAGA TGAACCATAG CATGCTTCAG AAGGCCCGAG 841 ACATGTATGC AGAGGAGCGG AAGAGGCAGC AGCTGGAGAG GGACCAGGCT ACAGTGACAG 901 AGCAGCTGCT GCGAGAGGGG CTCCAAGCCA GTGGGGACGC CCAGCTCCGA AGGACACGCT 961 TGCACAAACT CTCGGCCAGA CGGGAAGAGC GAGTCCAAGG CTTCCTGCAG GCCTTGGAAC 1021 TCAAGCGAGC TGACTGGCTG GCCCGTCTGG GCACTGCATC AGCCTGCCCA ACTTTCTTGT 1081 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1141 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1201 GAAAGGACGA TGGGAAATCC CAAACACACA TTTGACGCGT TAAGTCgaca atcaacctct 1261 ggattacaaa atttgtgaaa gatt