Construct: ORF TRCN0000471441
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009532.1_s317c1
- Derived from:
- ccsbBroadEn_06656
- DNA Barcode:
- ATACACAAGCTCGTTACTATTCCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- NPPA (4878)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471441
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4878 | NPPA | natriuretic peptide A | NM_006172.4 | 98.4% | 56.1% | 237delG;453_454insCGAAGA |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 396
- ORF length:
- 330
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ctccttctcc accaccaccg tgagcttcct ccttttactg gcattccagc 121 tcctaggtca gaccagagct aatcccatgt acaatgccgt gtccaacgca gacctgatgg 181 atttcaagaa tttgctggac catttggaag aaaagatgcc tttagaagat gaggtcgtgc 241 ccccacaagt gctcagtgag ccgaatgaag aagcgggggc tgctctcagc cccctcccTG 301 AGTGCCTCCC TGGACCGGGG AAGTCAGCCC AGCCCAGAGA GATGGAGGTG CCCTCGGGCG 361 GGGCCCCTGG GACTCCTCTG ATCGATCTGC CCTCCTAAAA AGCAAGCTGA GGGCGCTGCT 421 CACTGCCCCT CGGAGCCTGC GGAGATCCAG CTGCTTCGGG GGCAGGATGG ACAGGATTGG 481 AGCCCAGAGC GGACTGGGCT GTAACAGCTT CCGGTACCGA AGATACCCAA CTTTCTTGTA 541 CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA 601 GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG 661 AAAGGACGAA TACACAAGCT CGTTACTATT CCTACGCGTT AAGTCgacaa tcaacctctg 721 gattacaaaa tttgtgaaag att