Construct: ORF TRCN0000471445
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011352.1_s317c1
- Derived from:
- ccsbBroadEn_02798
- DNA Barcode:
- CGGCTAGTCCCACGTTATGAGCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARL2BP (23568)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471445
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23568 | ARL2BP | ADP ribosylation factor lik... | NM_012106.4 | 100% | 100% | |
| 2 | mouse | 107566 | Arl2bp | ADP-ribosylation factor-lik... | NM_024191.2 | 88.1% | 95.7% | (many diffs) |
| 3 | mouse | 107566 | Arl2bp | ADP-ribosylation factor-lik... | NM_024269.4 | 82% | 88.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 555
- ORF length:
- 489
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgccttagaa ggagagagct ttgcgctgtc tttctcctcc gcctctgatg 121 cagaatttga tgctgtggtt GGATATTTAG AGGACATTAT CATGGATGAC GAGTTCCAGT 181 TATTACAGAG AAATTTCATG GACAAGTACT ACCTGGAGTT TGAAGACACA GAAGAGAATA 241 AACTCATCTA CACACCTATT TTTAATGAAT ACATTTCTTT GGTAGAAAAA TACATTGAAG 301 AACAGCTGCT GCAGCGGATT CCTGAGTTCA ACATGGCAGC CTTCACCACA ACATTACAGC 361 ACCATAAGGA TGAAGTGGCT GGTGACATAT TCGACATGCT GCTCACCTTC ACAGATTTTC 421 TGGCTTTTAA AGAAATGTTT TTGGACTACA GAGCAGAAAA AGAAGGCCGA GGACTGGACT 481 TAAGCAGTGG CTTAGTGGTG ACTTCATTGT GCAAATCATC TTCTCTGCCA GCTTCCCAGA 541 ACAATCTGCG GCACTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 601 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 661 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CGGCTAGTCC CACGTTATGA 721 GCAAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt