Construct: ORF TRCN0000471489
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007760.1_s317c1
- Derived from:
- ccsbBroadEn_12679
- DNA Barcode:
- TGCACCTAGTGGAAAACTCCAGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RUBCNL (80183)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471489
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80183 | RUBCNL | rubicon like autophagy enha... | NM_001286763.3 | 99.9% | 99.8% | 253G>A |
| 2 | human | 80183 | RUBCNL | rubicon like autophagy enha... | NM_001286764.3 | 88.5% | 88.4% | 0_1ins204;49G>A |
| 3 | human | 80183 | RUBCNL | rubicon like autophagy enha... | NM_001286761.2 | 88.1% | 86.2% | (many diffs) |
| 4 | human | 80183 | RUBCNL | rubicon like autophagy enha... | NM_001349772.2 | 88.1% | 86.2% | (many diffs) |
| 5 | human | 80183 | RUBCNL | rubicon like autophagy enha... | NM_025113.4 | 88.1% | 86.2% | (many diffs) |
| 6 | human | 80183 | RUBCNL | rubicon like autophagy enha... | NM_001286765.2 | 84.8% | 84.8% | 63_64ins270 |
| 7 | human | 80183 | RUBCNL | rubicon like autophagy enha... | NM_001286762.3 | 77.6% | 67.8% | (many diffs) |
| 8 | human | 80183 | RUBCNL | rubicon like autophagy enha... | NM_001286766.2 | 75.1% | 75.1% | 0_1ins444 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1851
- ORF length:
- 1785
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ttccaatcat tactttctcc tctgtgtcaa tctgcctctt cgagaaattc 121 atactcctgg cccttcacct tcgtgcctcg gggactccct ggcagagaca acgttgtctg 181 aggataccac agactccgtt ggcagcgctt ctccccatgg ctcgagtgaa aagagtagca 241 gcttctctct gtcctcaaca gaggtacaca tggtccgccc aggatactct catcgggtgt 301 ctctgcccac aagccctagg attttggcca cctccccata tcctgagact gacagtgctt 361 tttttgagcc ttcccatctg acatctgctg ctgatgaagg tgctgttcaa gtcagtagaa 421 gaaccatttc ttcgaattcc ttctcaccag aggtatttgt gctgcctgtt gatgtagaaa 481 aggaaaatgc ccacttttat gttgcagata tgattatatc agcaatggag aaaatgaagt 541 gtaacattct gagtcaacag cagacagaga gctggagtaa agaagtcagt gggttacttg 601 ggagtgatca gcctgactct gaaatgactt ttgataccaa cataaagcaa gagtctgggt 661 cttctacttc ttcatacagt ggctatgaag gttgtgctgt gttacaggtc agcccagtga 721 ctgaaacacg tacttaccat gatgtgaaag agatttgcaa atgcgatgtt gatgaatttg 781 ttattttaga gcttggagat tttaatgata tcacagaaac ctgtagctgt tcctgcagct 841 cctctaagag tgtcacttat gagccagact tcaattctgc agaactatta gccaaagagc 901 tgtaccgcgt gttccagaag tgctggatac tgtcagtagt taattctcag ctggcaggtt 961 ccctgagtgc agctggctcg atagtcgtaa atgaagagtg tgtccgaaaa gactttgaat 1021 ccagtatgaa tgtagtacag gaaattaaat ttaagtctag gatcagaggg actgaagact 1081 gggctcctcc tagatttcaa atcatattta atattcatcc accactcaag agggaccttg 1141 tggtggcagc ccagaatttt ttctgtgccg gctgtggaac tccagtagag cctaagtttg 1201 tgaagcggct ccggtactgc gaatacctag ggaagtattt ctgtgactgc tgccactcat 1261 atgcagagtc gtgcatccct gcccgaatcc tgatgatgtg ggacttcaag aagtactacg 1321 tcagcaattt ctccaaacag ctgctcgaca gcatatggCA CCAGCCCATT TTCAATTTGC 1381 TGAGCATCGG CCAAAGCCTG TATGCGAAAG CCAAGGAGCT GGACAGAGTG AAGGAAATTC 1441 AGGAGCAGCT CTTCCATATC AAGAAGCTGT TGAAGACCTG TAGGTTTGCT AACAGTGCAT 1501 TAAAGGAGTT CGAGCAGGTG CCGGGACACT TGACTGATGA GCTCCACCTG TTCTCCCTTG 1561 AGGACCTGGT CAGGATCAAG AAAGGGCTGC TGGCACCCTT ACTCAAGGAC ATTCTGAAAG 1621 CTTCCCTTGC ACATGTGGCT GGCTGTGAGC TGTGTCAAGG AAAGGGCTTT ATTTGTGAAT 1681 TTTGCCAGAA TACGACTGTC ATCTTCCCAT TTCAGACAGC AACATGTAGA AGATGTTCAG 1741 CGTGCAGGGC TTGCTTTCAC AAACAGTGCT TCCAGTCCTC CGAGTGCCCC CGGTGTGCGA 1801 GGATCACAGC GAGGAGAAAA CTTCTGGAAA GTGTGGCCTC TGCAGCAACA TGCCCAACTT 1861 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1921 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1981 CTTGTGGAAA GGACGATGCA CCTAGTGGAA AACTCCAGCT ACGCGTTAAG TCgacaatca 2041 acctctggat tacaaaattt gtgaaagatt