Construct: ORF TRCN0000471496
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017427.1_s317c1
- Derived from:
- ccsbBroadEn_07074
- DNA Barcode:
- TTGACCCATGTAATCCAAAATCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TLR2 (7097)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471496
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7097 | TLR2 | toll like receptor 2 | NM_001318787.2 | 99.9% | 100% | 597T>C |
| 2 | human | 7097 | TLR2 | toll like receptor 2 | NM_001318789.2 | 99.9% | 100% | 597T>C |
| 3 | human | 7097 | TLR2 | toll like receptor 2 | NM_001318790.2 | 99.9% | 100% | 597T>C |
| 4 | human | 7097 | TLR2 | toll like receptor 2 | NM_001318791.2 | 99.9% | 100% | 597T>C |
| 5 | human | 7097 | TLR2 | toll like receptor 2 | NM_001318793.2 | 99.9% | 100% | 597T>C |
| 6 | human | 7097 | TLR2 | toll like receptor 2 | NM_001318795.2 | 99.9% | 100% | 597T>C |
| 7 | human | 7097 | TLR2 | toll like receptor 2 | NM_001318796.2 | 99.9% | 100% | 597T>C |
| 8 | human | 7097 | TLR2 | toll like receptor 2 | NM_003264.5 | 99.9% | 100% | 597T>C |
| 9 | human | 7097 | TLR2 | toll like receptor 2 | XM_011532215.2 | 99.9% | 100% | 597T>C |
| 10 | human | 7097 | TLR2 | toll like receptor 2 | XM_011532216.2 | 99.9% | 100% | 597T>C |
| 11 | human | 7097 | TLR2 | toll like receptor 2 | XM_017008573.1 | 99.9% | 100% | 597T>C |
| 12 | human | 7097 | TLR2 | toll like receptor 2 | XM_017008574.1 | 99.9% | 100% | 597T>C |
| 13 | human | 7097 | TLR2 | toll like receptor 2 | XM_017008575.1 | 99.9% | 100% | 597T>C |
| 14 | human | 7097 | TLR2 | toll like receptor 2 | XM_017008576.1 | 99.9% | 100% | 597T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2418
- ORF length:
- 2352
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc acatactttg tggatggtgt gggtcttggg ggtcatcatc agcctctcca 121 aggaagaatc ctccaatcag gcttctctgt cttgtgaccg caatggtatc tgcaagggca 181 gctcaggatc tttaaactcc attccctcag ggctcacaga agctgtaaaa agccttgacc 241 tgtccaacaa caggatcacc tacattagca acagtgacct acagaggtgt gtgaacctcc 301 aggctctggt gctgacatcc aatggaatta acacaataga ggaagattct ttttcttccc 361 tgggcagtct tgaacattta gacttatcct ataattactt atctaattta tcgtcttcct 421 ggttcaagcc cctttcttct ttaacattct taaacttact gggaaatcct tacaaaaccc 481 taggggaaac atctcttttt tctcatctca caaaattgca aatcctgaga gtgggaaata 541 tggacacctt cactaagatt caaagaaaag attttgctgg acttaccttc cttgaggaac 601 ttgagattga tgcttcagat ctacagagct atgagccaaa aagtttgaag tcaattcaga 661 acgtaagtca tctgatcctt catatgaagc agcatatttt actgctggag atttttgtag 721 atgttacaag ttccgtggaa tgtttggaac tgcgagatac tgatttggac actttccatt 781 tttcagaact atccactggt gaaacaaatt cattgattaa aaagtttaca tttagaaatg 841 tgaaaatcac cgatgaaagt ttgtttcagg ttatgaaact tttgaatcag atttctggat 901 tgttagaatt agagtttgat gactgtaccc ttaatggagt tggtaatttt agagcatctg 961 ataatgacag agttatagat ccaggtaaag tggaaacgtt aacaatccgg aggctgcata 1021 ttccaaggtt ttacttattt tatgatctga gcactttata ttcacttaca gaaagagtta 1081 aaagaatcac agtagaaaac agtaaagttt ttctggttcc ttgtttactt tcacaacatt 1141 taaaatcatt agaatacttg gatctcagtg aaaatttgat ggttgaagaa tacttgaaaa 1201 attcagcctg tgaggatgcc tggccctctc tacaaacttt aattttaagg caaaatcatt 1261 tggcatcatt ggaaaaaacc ggagagactt tgctcactct gaaaaacttg actaacattg 1321 atatcagtaa gaatagtttt cattctatgc ctgaaacttg tcagtggcca gaaaagatga 1381 aatatttgaa cttatccagc acacgaatac acagtgtaac aggctgcatt cccaagacac 1441 tggaaatttt agatgttagc aacaacaatc tcaatttatt ttctttgaat ttgccgcaac 1501 tcaaagaact ttatatttcc agaaataagt tgatgactct accagatgcc tccctcttac 1561 ccatgttact agtattgaaa atcagtagga atgcaataac tacgttttct aaggagcaac 1621 ttgactcatt tcacacactg aagactttgg aagctggtgg caataacttc atttgctcct 1681 gtgaattcct ctccttcact caggagcagc aagcactggc caaagtcttg attgattggc 1741 cagcaaatta cctgtgtgac tctccatccc atgtgcgtgg ccagcaggtt caggatgtcc 1801 gcctctcggt gtcggaatgt cacaggacag cactggtgtc tggcatgtgc tgtgctctgt 1861 tcctgctgat cctgctcacg ggggtcctgt gccaccgttt ccatggcctG TGGTATATGA 1921 AAATGATGTG GGCCTGGCTC CAGGCCAAAA GGAAGCCCAG GAAAGCTCCC AGCAGGAACA 1981 TCTGCTATGA TGCATTTGTT TCTTACAGTG AGCGGGATGC CTACTGGGTG GAGAACCTTA 2041 TGGTCCAGGA GCTGGAGAAC TTCAATCCCC CCTTCAAGTT GTGTCTTCAT AAGCGGGACT 2101 TCATTCCTGG CAAGTGGATC ATTGACAATA TCATTGACTC CATTGAAAAG AGCCACAAAA 2161 CTGTCTTTGT GCTTTCTGAA AACTTTGTGA AGAGTGAGTG GTGCAAGTAT GAACTGGACT 2221 TCTCCCATTT CCGTCTTTTT GATGAGAACA ATGATGCTGC CATTCTCATT CTTCTGGAGC 2281 CCATTGAGAA AAAAGCCATT CCCCAGCGCT TCTGCAAGCT GCGGAAGATA ATGAACACCA 2341 AGACCTACCT GGAGTGGCCC ATGGACGAGG CTCAGCGGGA AGGATTTTGG GTAAATCTGA 2401 GAGCTGCGAT AAAGTCCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2461 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2521 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTGACCC ATGTAATCCA 2581 AAATCCTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt