Construct: ORF TRCN0000471498
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006528.1_s317c1
- Derived from:
- ccsbBroadEn_10454
- DNA Barcode:
- CGGTGAGGTTTGCTCAGGCCGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BTN2A2 (10385)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471498
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_001197237.2 | 100% | 100% | |
| 2 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_006995.5 | 100% | 100% | |
| 3 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_005248797.3 | 100% | 100% | |
| 4 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_006714953.4 | 100% | 100% | |
| 5 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_011514228.2 | 100% | 100% | |
| 6 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_011514229.2 | 88.1% | 88.1% | 439_440ins186 |
| 7 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_017010166.2 | 88.1% | 88.1% | 439_440ins186 |
| 8 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_024446302.1 | 88.1% | 88.1% | 439_440ins186 |
| 9 | human | 11120 | BTN2A1 | butyrophilin subfamily 2 me... | NM_007049.5 | 85% | 79.4% | (many diffs) |
| 10 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_181531.3 | 77.8% | 77.8% | 91_92ins348 |
| 11 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_005248798.4 | 77.8% | 77.8% | 91_92ins348 |
| 12 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_017010167.1 | 77.8% | 77.8% | 91_92ins348 |
| 13 | human | 11120 | BTN2A1 | butyrophilin subfamily 2 me... | NM_001197233.2 | 74.5% | 69.3% | (many diffs) |
| 14 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_006714955.3 | 65.5% | 60.5% | (many diffs) |
| 15 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_017010168.2 | 65.5% | 60.5% | (many diffs) |
| 16 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_017010169.1 | 65.5% | 60.5% | (many diffs) |
| 17 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_001197238.2 | 63% | 62.8% | (many diffs) |
| 18 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_001197239.2 | 59.8% | 59.8% | 91_92ins630 |
| 19 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | XM_011514231.3 | 59.8% | 59.8% | 91_92ins630 |
| 20 | human | 11120 | BTN2A1 | butyrophilin subfamily 2 me... | NM_078476.4 | 51.9% | 47.9% | (many diffs) |
| 21 | human | 11120 | BTN2A1 | butyrophilin subfamily 2 me... | NM_001197234.3 | 51.3% | 47.5% | (many diffs) |
| 22 | human | 10385 | BTN2A2 | butyrophilin subfamily 2 me... | NM_001197240.2 | 47.4% | 45.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1635
- ORF length:
- 1569
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga accagctgct gctctgcact tctccctgcc agcctccctc ctcctcctcc 121 tgctcctcct ccttctcagc ctgtgtgcac tggtctcagc ccagtttact gtcgtggggc 181 cagctaatcc catcctggcc atggtgggag aaaacactac attacgctgc catctgtcac 241 ccgagaaaaa tgctgaggac atggaggtgc ggtggttccg gtctcagttc tcccccgcag 301 tgtttgtgta taagggtggg agagagagaa cagaggagca gatggaggag taccggggaa 361 gaatcacctt tgtgagcaaa gacatcaaca ggggcagcgt ggccctggtc atacataacg 421 tcacagccca ggagaatggg atctaccgct gttacttcca agaaggcagg tcctacgatg 481 aggccatcct acgcctcgtg gtggcaggcc ttgggtctaa gcccctcatt gaaatcaagg 541 cccaagagga tgggagcatc tggctggagt gcatatctgg agggtggtac ccagagcccc 601 tcacagtgtg gagggacccc tacggtgagg ttgtgcccgc cctgaaggag gtttccatcg 661 ctgatgctga cggcctcttc atggtcacca cagctgtgat catcagagac aagtatgtga 721 ggaatgtgtc ctgctctgtc aacaacaccc tgctcggcca ggagaaggaa actgtcattt 781 ttattccaga atcctttatg cccagcgcat ctccctggat ggtggcccta gctgtcatcc 841 tgaccgcatc tccctggatg gtgtccatga ctgtcatcct ggctgttttc atcatcttca 901 tggctgtcag catctgttgc atcaagaaac ttcaaaggga aaaaaagatt ctgtcagggg 961 aaaagaaagt tgaacaagag gaaaaagaaa ttgcacagca acttcaagaa gaattgcgat 1021 ggagaagaac attcttacat gctgctgatg tggtcctgga tccagacacc gctcatcccg 1081 agctcttcct gtcagaggac cggagaagtg tgaggcgggg cccctacagg cagagagtgc 1141 ctgacaaccc agagagattc gacagtcagc cttgtgtccT GGGATGGGAG AGCTTCGCCT 1201 CAGGGAAACA TTACTGGGAG GTGGAGGTGG AAAACGTGAT GGTGTGGACT GTGGGGGTCT 1261 GCAGACACAG TGTTGAGAGG AAAGGGGAGG TCCTGCTGAT TCCTCAGAAT GGCTTCTGGA 1321 CCCTGGAGAT GTTTGGAAAC CAATACCGGG CCCTGTCCTC CCCTGAGAGG ATTCTCCCTT 1381 TGAAGGAGTC CCTTTGCCGG GTGGGCGTCT TCCTGGACTA TGAAGCTGGA GATGTCTCCT 1441 TCTACAACAT GAGGGACAGA TCGCACATCT ACACATGTCC CCGTTCAGCC TTTACTGTGC 1501 CTGTGAGGCC CTTCTTCAGG TTAGGGTCTG ATGACAGCCC CATCTTCATC TGCCCTGCAC 1561 TCACAGGAGC CAGTGGGGTC ATGGTGCCTG AAGAGGGCCT GAAACTTCAC AGAGTGGGGA 1621 CCCACCAGAG CCTATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1681 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1741 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CGGTGAGGTT TGCTCAGGCC 1801 GTCAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt