Construct: ORF TRCN0000471499
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002515.1_s317c1
- Derived from:
- ccsbBroadEn_08093
- DNA Barcode:
- CAATAATTTAATCTCGTCGAGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCPS (28960)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471499
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28960 | DCPS | decapping enzyme, scavenger | NM_014026.6 | 99.7% | 99.7% | 218G>A;315G>A;480T>C |
| 2 | human | 28960 | DCPS | decapping enzyme, scavenger | NM_001350236.2 | 97.6% | 97.6% | (many diffs) |
| 3 | mouse | 69305 | Dcps | decapping enzyme, scavenger | NM_027030.2 | 85.6% | 88.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1077
- ORF length:
- 1011
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggacgcagct cctcaactag gcaagaggaa gcgcgaattg gacgtggagg 121 aggcccacgc cgccagcaca gaggaaaagg aggcaggagt tggaaatggt acctgtgctc 181 ctgtccgctt accgttctcc ggcttcagac tgcagaaggt gctgagggag tctgcgcggg 241 acaaaatcat tttcctacac gggaaggtga atgaggcctc tgaggatggg gatggagagg 301 atgccgttgt gatcctggag aagacgccat ttcaggtgga acaggtggct cagctcctga 361 cgggcagccc tgagctccaa ttgcagttct ccaatgatat ctacagcacc tatcacttgt 421 tccctccaag acaactgaat gatgtaaaga cgaccgtggt ttaccctgcc acagagaaac 481 acctgcagaa gtacctgcgc caggacctcc gcctgatccg agagacggga gatgactaca 541 ggaacattac tttaccccac ctggagtccc agagcctcag catccagtgg gtgtataaca 601 ttctcgacaa gaaggctgaa gcggaccgga ttgttttcga gaacccagat ccctctgatg 661 gttttgtcct catccctgac ctcaagtgga accaacagca gctcgatgac ttgtacttga 721 tcgccatctg ccatcgccgg ggcatcagat cccTACGCGA CCTTACTCCG GAGCACTTGC 781 CGCTGCTCAG GAACATCCTC CACCAGGGGC AGGAGGCCAT CCTGCAGCGC TACCGGATGA 841 AGGGAGACCA TCTGCGAGTA TACCTGCACT ACCTGCCCTC CTACTACCAC CTGCATGTGC 901 ACTTCACCGC CCTGGGCTTC GAGGCCCCCG GCTCAGGCGT GGAGCGGGCC CACCTGCTGG 961 CTGAGGTGAT CGAGAACTTG GAGTGTGACC CTAGGCACTA CCAGCAGCGC ACGCTCACCT 1021 TCGCCCTCAG GGCTGACGAC CCCCTGCTCA AGCTCTTGCA GGAGGCTCAG CAAAGCTGCC 1081 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1141 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1201 ATATATCTTG TGGAAAGGAC GACAATAATT TAATCTCGTC GAGATCACGC GTTAAGTCga 1261 caatcaacct ctggattaca aaatttgtga aagatt