Construct: ORF TRCN0000471502
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016473.1_s317c1
- Derived from:
- ccsbBroadEn_13955
- DNA Barcode:
- AGCCCACGGATCGAGCTGAATATT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SMTN (6525)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471502
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6525 | SMTN | smoothelin | NM_134269.3 | 99.9% | 58.3% | 1598_1599insN;1639G>C |
| 2 | human | 6525 | SMTN | smoothelin | NM_006932.5 | 97.1% | 58.2% | (many diffs) |
| 3 | human | 6525 | SMTN | smoothelin | NM_134270.3 | 96% | 56.8% | (many diffs) |
| 4 | human | 6525 | SMTN | smoothelin | XM_017028915.1 | 94.3% | 55.1% | 1_162del;1760_1761insN;1801G>C |
| 5 | human | 6525 | SMTN | smoothelin | XM_006724298.1 | 93.9% | 56.3% | (many diffs) |
| 6 | human | 6525 | SMTN | smoothelin | NM_001207018.1 | 93.3% | 53.6% | (many diffs) |
| 7 | human | 6525 | SMTN | smoothelin | XM_017028914.1 | 91.7% | 54.9% | (many diffs) |
| 8 | human | 6525 | SMTN | smoothelin | XM_011530333.3 | 91.4% | 53.4% | (many diffs) |
| 9 | human | 6525 | SMTN | smoothelin | NM_001207017.1 | 88.9% | 53.2% | (many diffs) |
| 10 | human | 6525 | SMTN | smoothelin | XM_017028913.1 | 88.9% | 53.2% | (many diffs) |
| 11 | human | 6525 | SMTN | smoothelin | XM_017028916.1 | 86.9% | 55.9% | (many diffs) |
| 12 | human | 6525 | SMTN | smoothelin | XM_017028919.1 | 86.5% | 59.7% | (many diffs) |
| 13 | human | 6525 | SMTN | smoothelin | XM_006724302.1 | 84.7% | 47% | (many diffs) |
| 14 | human | 6525 | SMTN | smoothelin | XM_011530338.2 | 83.9% | 59.6% | (many diffs) |
| 15 | human | 6525 | SMTN | smoothelin | XM_011530337.2 | 83.8% | 57.7% | (many diffs) |
| 16 | human | 6525 | SMTN | smoothelin | XM_017028917.1 | 83.4% | 57.6% | (many diffs) |
| 17 | human | 6525 | SMTN | smoothelin | XM_011530336.2 | 81.4% | 57.6% | (many diffs) |
| 18 | human | 6525 | SMTN | smoothelin | XM_017028918.1 | 71.7% | 57.9% | (many diffs) |
| 19 | human | 6525 | SMTN | smoothelin | XM_011530339.2 | 63.7% | 73% | (many diffs) |
| 20 | human | 6525 | SMTN | smoothelin | XR_002958712.1 | 61% | (many diffs) | |
| 21 | human | 6525 | SMTN | smoothelin | XM_011530341.1 | 58.1% | 20.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1674
- ORF length:
- 1608
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggacgaggcc ttagctgggc tggatgaggg agcccttcgg aagctgctgg 121 aggtcacagc agatctggca gagcggcggc gcatccgctc agccatccgg gaactgcagc 181 ggcaggagct ggagcgcgag gaggaggccc tggcatccaa gcgtttccgt gccgagcggc 241 aggacaacaa ggagaactgg ctgcactctc agcagcggga agctgagcag cgggctgccc 301 tggcacggct ggcagggcag ctggagtcca tgaacgatgt ggaggaattg actgcactgt 361 tgcgaagcgc tggtgagtat gaggagcgca agctgatccg agctgccatc cgccgtgtac 421 gggctcagga gattgaggct gccaccttgg ctgggaggtt gtacagcggg cgtcccaaca 481 gtggctcaag agaggacagc aaggggctag cggcacacag gctggaacag tgtgaggtgc 541 cagagcgaga ggaacaggaa cagcaggcag aggtttcaaa gccaaccccc acccctgaag 601 gcaccagcca ggatgtgacc acagtgacac tcctgctgcg agccccacct gggagcacat 661 ccagctcacc tgcctcaccc agcagttcac ccacccctgc ctctcctgag cctccattgg 721 agcctgccga ggcccagtgc cttacagctg aggttccagg cagcccagag ccacccccca 781 gcccacccaa gaccaccagc cctgagcctc aggagtctcc aacgctcccc agcactgagg 841 gccaggtggt caacaagctt ctgtctggcc ccaaagagac ccctgctgcc cagagcccca 901 ccagaggccc ctctgacacc aagagagcag acgtggctgg accccgaccc tgccaacgct 961 ccctgtcggt gctcagcccc cgccaaccag cccagaaccg agagtccacc ccccttgcca 1021 gcggaccttc ctcattccag cgggctggct ctgtgcggga tcgtgtccac aagttcacat 1081 ctgattctcc tatggctgct aggctccagg atggcacacc ccaggctgcc ctaagtcccc 1141 tgacccccgc aaggctcctg ggcccctccc tcaccagcac cacccctgcc tcctcctcca 1201 gcggctcctc ctctcggggc cccagtgata cctcctcccg gttcagcaag gagcaacgag 1261 gagtagccca gcccctggcc cagcttcgaa gctgccccca ggaggagggc cccagggggc 1321 ggggcttggc tgctaggccc cttgaaaaca gagcaggggg gcctgtggca cgttcagagg 1381 agcctggtgc cccgctgccc gtggccgtcg gcactgccga gccagggggc agtatgaaga 1441 ccacattcac catcgagatc aaggacggcc gtggccaggc ctccacaggc cgggtgctgc 1501 tgcccacagg caaccagagg gcagaactga cactggggct gcgggcgccc ccgaccctac 1561 tcagcaccag tagtgggggc aagagcacca tcacccgtgt caacagccct gggaccctgg 1621 ctcggctggg cagtgtcact catgtcacca gcttcagcca tgcncccccc cagtagccga 1681 ggaggctgca gcatcaagat ggaaccagag ccagcagagc ctctcgctgc agcagtggaa 1741 gcggccaatg gggctgagca gacccgagtg aacaaagcac cagaagggcg gagccctctg 1801 agcgctgagg agctgatgac tattgaggat gaaggagtct tggacaagat gctggatcag 1861 agcacggact ttgaagagcg gaagctcatc cgggctgcac ttcgtgagct ccgacaaagg 1921 aagagagacc agcgggacaa ggagcgggaa cggcggctgc aggaggcacg gggccggcca 1981 ggggaggggc gcggcaacac agccactgag accaccacga ggcacagcca gcgggcagct 2041 gatggctctg ctgtcagcac tgttaccaag actgagcggc tcgtccactc caatgatggc 2101 acacggacgg cccgcaccac cacagtggag tcgagtttcg tgaggcgctc ggagaatggc 2161 agtggcagca ccatgatgca aaccaagacc ttctcctctt cctcctcatc caagaagatg 2221 ggcagcatct tcgaccgcga ggaccaggcc agcccacggg ccggcagcct ggcggcgctc 2281 gagaaacggc aggccgagaa gaagaaagag ctgatgaagg cgcagagtct gcccaagacc 2341 tcagcctccc aggcgcgcaa ggccatgatt gagaagctgg agaaggaggg cgcggccggc 2401 agccctggcg gaccccgcgc agccgtgcag cgatccacca gcttcggggt ccccaacgcc 2461 aacagcatca agcagatgct gctggactgg tgtcgagcca agactcgcgg ctacgagcac 2521 gtcgacatcc agaacttctc ctccagctgg agtgatggga tggccttctg tgccctggtg 2581 cacaacttct tccctgaggc cttcgactat gggcagctta gccctcagaa ccgacgccag 2641 aacttcgagg tggccttctc atctgcggag acccatgcgg actgcccgca gctcctggat 2701 acagaggaca tggtgcggct tcgagagcct gactggaagt gcgtgtacac gtacatccaG 2761 GAATTCTACC GCTGTCTGGT CCAGAAGGGG CTGGTAAAAA CCAAAAAGTC CTACCCAACT 2821 TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT 2881 TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA 2941 TCTTGTGGAA AGGACGAAGC CCACGGATCG AGCTGAATAT TACGCGTTAA GTCgacaatc 3001 aacctctgga ttacaaaatt tgtgaaagat t