Construct: ORF TRCN0000471507
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004393.1_s317c1
- Derived from:
- ccsbBroadEn_06538
- DNA Barcode:
- TTTTATCACACATTAGATGGCATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LSP1 (4046)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471507
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4046 | LSP1 | lymphocyte specific protein 1 | NM_002339.3 | 99.8% | 99.7% | 30C>T;298G>A |
| 2 | human | 4046 | LSP1 | lymphocyte specific protein 1 | NM_001013253.2 | 81.6% | 81.4% | 0_1ins186;112G>A |
| 3 | human | 4046 | LSP1 | lymphocyte specific protein 1 | NM_001013254.1 | 81.6% | 81.4% | 0_1ins186;112G>A |
| 4 | human | 4046 | LSP1 | lymphocyte specific protein 1 | NM_001013255.1 | 81.6% | 81.4% | 0_1ins186;112G>A |
| 5 | human | 4046 | LSP1 | lymphocyte specific protein 1 | NM_001289005.1 | 81.6% | 81.4% | 0_1ins186;112G>A |
| 6 | human | 4046 | LSP1 | lymphocyte specific protein 1 | NM_001242932.1 | 71.3% | 68.7% | (many diffs) |
| 7 | human | 654342 | LSP1P4 | LSP1 pseudogene 4 | NR_027238.1 | 22.2% | (many diffs) | |
| 8 | mouse | 16985 | Lsp1 | lymphocyte specific 1 | NM_001271523.1 | 20% | 21.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1083
- ORF length:
- 1017
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggaggcttcg agtgacccgg gtgctgagga gcgggaagag ttgctggggc 121 ccactgctca gtggagcgtg gaggacgagg aggaggccgt ccacgagcaa tgccagcatg 181 agagagacag gcagcttcag gcccaggacg aggagggagg cggccatgtc cccgagcggc 241 cgaagcagga gatgctcctc agcctgaagc cctcggaggc ccctgaactg gatgaggacg 301 agggctttgg cgactggtcc cagaggccag agcagcggca gcagcacgag ggggcgcagg 361 gcaccttgga cagcggagag cccccccagt gcaggagtcc tgagggggag caagaggaca 421 ggcccggcct gcatgcctac gaaaaggagg acagtgatga agtccacctg gaggagttga 481 gtctgagcaa ggaggggcca ggcccagagg acactgtcca ggacaacctg ggggccgcag 541 gggctgagga ggaacaggag gagcaccaga aatgtcagca gcccaggaca cccagcccct 601 tggtcttgga ggggaccatc gaacagagct cgcctccccT GAGCCCTACC ACCAAACTCA 661 TCGACAGGAC CGAGTCCCTA AACCGCTCCA TAGAGAAGAG TAACAGTGTG AAGAAATCCC 721 AGCCAGACTT GCCCATCTCC AAGATTGATC AGTGGCTGGA ACAATACACC CAGGCCATCG 781 AGACCGCTGG CCGGACCCCC AAGCTAGCCC GCCAGGCCTC CATAGAGCTG CCCAGCATGG 841 CTGTGGCCAG TACCAAGAGT CGGTGGGAGA CGGGTGAGGT ACAGGCTCAG TCTGCGGCCA 901 AGACTCCGTC CTGCAAGGAT ATTGTGGCTG GAGACATGAG CAAGAAAAGC CTCTGGGAGC 961 AGAAGGGAGG CTCCAAGACC TCATCAACAA TTAAGAGCAC CCCATCTGGG AAGAGGTATA 1021 AGTTTGTGGC CACCGGGCAT GGGAAGTATG AGAAGGTGCT TGTGGAAGGG GGCCCGGCTC 1081 CCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1141 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1201 GGCTTTATAT ATCTTGTGGA AAGGACGATT TTATCACACA TTAGATGGCA TCACGCGTTA 1261 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt