Transcript: Human NM_001013255.1

Homo sapiens lymphocyte specific protein 1 (LSP1), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
LSP1 (4046)
Length:
1578
CDS:
184..1017

Additional Resources:

NCBI RefSeq record:
NM_001013255.1
NBCI Gene record:
LSP1 (4046)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001013255.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000412822 AGCTCCGCTTCTTGTTACTTG pLKO_005 1254 3UTR 100% 4.950 6.930 N LSP1 n/a
2 TRCN0000117220 CGAGTCCCTAAACCGCTCCAT pLKO.1 603 CDS 100% 0.880 1.232 N LSP1 n/a
3 TRCN0000430058 ACAATACACCCAGGCCATCGA pLKO_005 693 CDS 100% 2.640 2.112 N LSP1 n/a
4 TRCN0000117218 CCCATCTGGGAAGAGGTATAA pLKO.1 933 CDS 100% 13.200 9.240 N LSP1 n/a
5 TRCN0000117219 ACCGCTCCATAGAGAAGAGTA pLKO.1 614 CDS 100% 4.950 3.465 N LSP1 n/a
6 TRCN0000415504 AGAAGAGTAACAGTGTGAAGA pLKO_005 626 CDS 100% 4.950 3.465 N LSP1 n/a
7 TRCN0000412440 GCCCATCTCCAAGATTGATCA pLKO_005 663 CDS 100% 4.950 3.465 N LSP1 n/a
8 TRCN0000117217 GCTAAGAAGTTGCTTCCTGTT pLKO.1 1115 3UTR 100% 4.050 2.835 N LSP1 n/a
9 TRCN0000413096 TGAAGTCCACCTGGAGGAGTT pLKO_005 390 CDS 100% 4.050 2.835 N LSP1 n/a
10 TRCN0000413499 GAGTTGAGTCTGAGCAAGGAG pLKO_005 406 CDS 100% 2.640 1.848 N LSP1 n/a
11 TRCN0000090969 GCTGGAGACATGAGCAAGAAA pLKO.1 859 CDS 100% 5.625 2.813 Y Lsp1 n/a
12 TRCN0000117221 GTCCACGAGCAATGCCAGCAT pLKO.1 91 5UTR 100% 0.880 0.440 Y LSP1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001013255.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06538 pDONR223 100% 81.6% 81.4% None 0_1ins186;112G>A n/a
2 ccsbBroad304_06538 pLX_304 0% 81.6% 81.4% V5 0_1ins186;112G>A n/a
3 TRCN0000471507 TTTTATCACACATTAGATGGCATC pLX_317 43.7% 81.6% 81.4% V5 0_1ins186;112G>A n/a
4 ccsbBroadEn_13744 pDONR223 100% 20.7% 17.9% None (many diffs) n/a
5 ccsbBroad304_13744 pLX_304 0% 20.7% 17.9% V5 (many diffs) n/a
6 TRCN0000478469 GAAAGTCCGTCCATTGTTCGGGAG pLX_317 74.3% 20.7% 17.9% V5 (many diffs) n/a
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