Construct: ORF TRCN0000471508
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010351.1_s317c1
- Derived from:
- ccsbBroadEn_03439
- DNA Barcode:
- TAGCTCCATCCCTTCTTTCTTACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNB1L (54584)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471508
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54584 | GNB1L | G protein subunit beta 1 like | NM_053004.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1047
- ORF length:
- 981
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ggccccctgc ccgccgccac ctccagaccc ccagtttgtc ctccgaggca 121 cccagtcacc ggtgcatgcg ctgcacttct gcgaaggagc ccaggctcag gggcgcccgc 181 tcctcttctc agggtctcag agtggcctgg tacacatctg gagcctgcag acgcggagag 241 cggttaccac cctggatggc cacggcggcc agtgtgtgac ctggctgcag acgctgcccc 301 aggggcgcca gctcctcagt cagggccggg acctgaagct gtgcctgtgg gacctcgcgg 361 agggcaggag cgctgtcgtg gactccgtgt gcttggagag tgtgggcttc tgccggagca 421 gcatcctggc cgggggccag ccacgctgga cgcttgccgt gccagggagg ggcagcgacg 481 aggttcagat tctggagatg ccctccaaga cgtcagtgtg cgccctgaag ccgaaggcag 541 atgccaagct gggcatgccc atgtgcctgc ggctgtggca ggccgactgc agctcccgcc 601 cactccTTCT GGCCGGCTAT GAGGATGGAT CGGTGGTCCT GTGGGACGTC TCTGAGCAGA 661 AGGTGTGCAG CCGCATCGCC TGCCATGAGG AGCCCGTCAT GGACCTTGAC TTTGACTCCC 721 AGAAGGCCAG GGGCATCTCA GGCTCCGCGG GGAAGGCGCT GGCTGTCTGG AGCCTGGACT 781 GGCAGCAGGC CCTGCAGGTG CGTGGGACTC ATGAACTCAC CAATCCCGGG ATCGCCGAGG 841 TCACGATCCG GCCAGATCGC AAGATCCTGG CCACCGCAGG CTGGGACCAC CGCATCCGCG 901 TGTTCCACTG GCGGACGATG CAGCCACTGG CCGTGCTGGC CTTCCACAGC GCCGCTGTCC 961 AGTGCGTGGC CTTCACCGCC GATGGCTTGC TGGCCGCGGG CTCCAAGGAT CAGCGGATCA 1021 GCCTCTGGTC ACTCTACCCA CGCGCATGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1081 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1141 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATAGCTCCA 1201 TCCCTTCTTT CTTACAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1261 aagatt