Construct: ORF TRCN0000471521
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006044.1_s317c1
- Derived from:
- ccsbBroadEn_08498
- DNA Barcode:
- GCTCTTTGGACTACAATACAGCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM39A (55254)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471521
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55254 | TMEM39A | transmembrane protein 39A | NM_018266.3 | 100% | 100% | |
| 2 | human | 55254 | TMEM39A | transmembrane protein 39A | XM_006713687.2 | 100% | 100% | |
| 3 | human | 55254 | TMEM39A | transmembrane protein 39A | XM_017006788.2 | 94.2% | 94.2% | 335_336ins84 |
| 4 | human | 55254 | TMEM39A | transmembrane protein 39A | XM_005247578.2 | 77.2% | 76.2% | 1110_1111ins121;1131_1132ins212 |
| 5 | human | 55254 | TMEM39A | transmembrane protein 39A | XM_011512958.3 | 64.6% | 63.5% | (many diffs) |
| 6 | human | 55254 | TMEM39A | transmembrane protein 39A | XR_001740197.2 | 32.5% | 1_371del;1484_1664del;2017_4500del | |
| 7 | human | 55254 | TMEM39A | transmembrane protein 39A | XR_001740198.2 | 30.6% | (many diffs) | |
| 8 | human | 55254 | TMEM39A | transmembrane protein 39A | NR_073506.2 | 29.5% | 1_354del;773_881del;1928_4962del | |
| 9 | mouse | 67846 | Tmem39a | transmembrane protein 39a | NM_001205286.1 | 89.8% | 95% | (many diffs) |
| 10 | mouse | 67846 | Tmem39a | transmembrane protein 39a | NM_026407.3 | 89.8% | 95% | (many diffs) |
| 11 | mouse | 67846 | Tmem39a | transmembrane protein 39a | XM_006522496.3 | 84.4% | 89.5% | (many diffs) |
| 12 | mouse | 67846 | Tmem39a | transmembrane protein 39a | NM_001205287.1 | 77.6% | 82.3% | (many diffs) |
| 13 | mouse | 67846 | Tmem39a | transmembrane protein 39a | XR_001781854.1 | 60.4% | (many diffs) | |
| 14 | mouse | 67846 | Tmem39a | transmembrane protein 39a | XR_384596.3 | 60% | (many diffs) | |
| 15 | mouse | 67846 | Tmem39a | transmembrane protein 39a | XM_006522497.3 | 57.2% | 59% | (many diffs) |
| 16 | mouse | 67846 | Tmem39a | transmembrane protein 39a | XR_001781853.1 | 42.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1530
- ORF length:
- 1464
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cggtggaagg aggggcccta gtcggcaaca gctaagccgt tcagctttac 121 cttctttgca gactttggtt ggtggaggct gtggcaatgg aacaggcttg agaaacagga 181 atggtagtgc tattggcctt ccagtcccac ctatcacagc cttaatcacc ccaggtcctg 241 ttcgtcattg ccaaattcct gacttgcctg tggatgggag cctactcttt gaattccttt 301 ttttcatcta cctgttggtt gctcttttca ttcagtacat caacatttat aaaacagtgt 361 ggtggtatcc ttacaatcat cctgcttctt gtacatcact gaattttcat ctcattgatt 421 atcatctggc agcattcatc acagtgatgc ttgcgaggag gcttgtatgg gctctcatct 481 cagaggctac taaggcaggt gcagcatcaa tgattcacta catggttctg atatcagctc 541 gcttggtact actcactttg tgtggatggg tactttgttg gaccctcgtc aatctctttc 601 gaagccattc agtcctcaat ctccttttcc ttggctaccc gtttggtgtt tatgttcctc 661 tttgctgttt tcatcaagat agtagagcac atcttcttct cacagactac aactatgtgg 721 ttcagcacga ggcagtagag gaaagtgcct cgactgtggg aggcttggcc aaatccaaag 781 actttctctc cttgttgctg gagtcgctaa aagaacagtt taataatgcc acacccatcc 841 ccacccacag ttgtccccta tctccagacc tcattcgcaa tgaagtagaa tgtctgaaag 901 cagatttcaa ccacagaatc aaggaggttc tcttcaactc cctcttcagt gcctactatg 961 ttgcatttct ccccctgtgt tttgtgaaga gtacccagta ctatgacatg cgctggtcat 1021 gtgagcacct cattatggtg tggatcaatg cttttgtcat gctcaccacg caactgttgc 1081 catccaaata ctgtgatttg ctacataaat cagctgctca cctgggcaag tggcagaagt 1141 tGGAACATGG GTCCTACAGC AATGCTCCAC AGCACATTTG GTCAGAAAAT ACAATATGGC 1201 CTCAAGGGGT GCTGGTGCGG CACAGCAGAT GTTTATATAG AGCCATGGGG CCTTACAACG 1261 TGGCAGTGCC TTCAGATGTA TCTCATGCCC GCTTTTATTT CTTATTTCAT CGACCATTAA 1321 GGCTGTTAAA TCTGCTCATC CTTATTGAGG GCAGTGTCGT CTTCTATCAG CTCTATTCCT 1381 TGCTGCGGTC GGAGAAGTGG AACCACACAC TTTCCATGGC TCTCATCCTC TTCTGCAACT 1441 ACTATGTTTT ATTTAAACTT CTCCGGGACA GAATAGTATT AGGCAGGGCA TACTCCTACC 1501 CACTCAACAG TTATGAACTC AAGGCAAACT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1561 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1621 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCTCT 1681 TTGGACTACA ATACAGCCCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1741 tgaaagatt