Construct: ORF TRCN0000471537
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004837.1_s317c1
- Derived from:
- ccsbBroadEn_00815
- DNA Barcode:
- TCCAGCAGACCAGCGGGTTTACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ID2 (3398)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471537
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3398 | ID2 | inhibitor of DNA binding 2 | NM_002166.5 | 100% | 100% | |
| 2 | mouse | 15902 | Id2 | inhibitor of DNA binding 2 | NM_010496.3 | 95% | 98.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 492
- ORF length:
- 402
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAAAGCCTT CAGTCCCGTG AGGTCCGTTA 121 GGAAAAACAG CCTGTCGGAC CACAGCCTGG GCATCTCCCG GAGCAAAACC CCTGTGGACG 181 ACCCGATGAG CCTGCTATAC AACATGAACG ACTGCTACTC CAAGCTCAAG GAGCTGGTGC 241 CCAGCATCCC CCAGAACAAG AAGGTGAGCA AGATGGAAAT CCTGCAGCAC GTCATCGACT 301 ACATCTTGGA CCTGCAGATC GCCCTGGACT CGCATCCCAC TATTGTCAGC CTGCATCACC 361 AGAGACCCGG GCAGAACCAG GCGTCCAGGA CGCCGCTGAC CACCCTCAAC ACGGATATCA 421 GCATCCTGTC CTTGCAGGCT TCTGAATTCC CTTCTGAGTT AATGTCAAAT GACAGCAAAG 481 CACTGTGTGG CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 541 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 601 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCC AGCAGACCAG CGGGTTTACA 661 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t