Construct: ORF TRCN0000471539
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007403.1_s317c1
- Derived from:
- ccsbBroadEn_07941
- DNA Barcode:
- ACTCCAACAGTGTCAACTCATGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBXO7 (25793)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471539
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25793 | FBXO7 | F-box protein 7 | NM_012179.4 | 99.8% | 99.8% | 345G>A;949C>T |
| 2 | human | 25793 | FBXO7 | F-box protein 7 | NM_001033024.1 | 84.1% | 82.1% | (many diffs) |
| 3 | human | 25793 | FBXO7 | F-box protein 7 | NM_001257990.1 | 78% | 77.9% | 0_1ins342;3G>A;607C>T |
| 4 | human | 25793 | FBXO7 | F-box protein 7 | XM_024452207.1 | 78% | 77.9% | 0_1ins342;3G>A;607C>T |
| 5 | human | 25793 | FBXO7 | F-box protein 7 | XM_011530106.1 | 70% | 70.1% | 0_1ins468;481C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1632
- ORF length:
- 1566
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gctgcgggtg cggcttctga agcggacctg gccgctggag gtgcccgaga 121 cggagccgac gctggggcat ttgcgctcgc acctgaggca gtccctgctg tgcacctggg 181 ggtacagttc taatacccga tttacaatta cattgaacta caaggatccc ctcactggag 241 atgaagagac cttggcttca tatgggattg tttctgggga cttgatatgt ttgattcttc 301 aagatgacat tccagcgcct aatatacctt catccacaga ttcagagcat tcttcactcc 361 agaataatga gcaaccctct ttggccacca gctccaatca gactagcata caggatgaac 421 aaccaagtga ttcattccaa ggacaggcag cccagtctgg tgtttggaat gacgacagta 481 tgttagggcc tagtcaaaat tttgaagctg agtcaattca agataatgcg catatggcag 541 agggcacagg tttctatccc tcagaaccca tgctctgtag tgaatcggtg gaagggcaag 601 tgccacattc attagagacc ttgtatcaat cagctgactg ttctgatgcc aatgatgcct 661 tgatagtgtt gatacatctt ctcatgttgg agtcaggtta catacctcag ggcaccgaag 721 ccaaagcact gtccatgccg gagaagtgga agttgagcgg ggtgtataag ctgcagtaca 781 tgcatcctct ctgcgagggc agctccgcta ctctcacctg tgtgcctttg ggaaacctga 841 ttgttgtaaa tgctacacta aaaatcaaca atgagattag aagtgtgaaa agattgcagc 901 tgctaccaga atcttttatt tgcaaagaga aactagggga aaatgtagcc aacatataca 961 aagatcttca gaaactctct cgcctcttta aagaccagct ggtgtatcct cttttggctt 1021 ttacccgaca agcactgaac ctaccagatg tatttgggtt ggtcgtcctc ccattggaac 1081 tgaaactacg gatcttccga cttctggatg ttcgttccgt cttgtctttg tctgcggttt 1141 gtcgtgacct ctttactgct tcaaatgacc cacTCCTGTG GAGGTTTTTA TATCTGCGTG 1201 ATTTTCGAGA CAATACTGTC AGAGTTCAAG ACACAGATTG GAAAGAACTG TACAGGAAGA 1261 GGCACATACA AAGAAAAGAA TCCCCGAAAG GGCGGTTTGT GATGCTCCTG CCATCGTCAA 1321 CTCACACCAT TCCATTCTAT CCCAACCCCT TGCACCCTAG GCCATTTCCT AGCTCCCGCC 1381 TTCCTCCAGG AATTATCGGG GGTGAATATG ACCAAAGACC AACACTTCCC TATGTTGGAG 1441 ACCCAATCAG TTCACTCATT CCTGGTCCTG GGGAGACGCC CAGCCAGTTT CCTCCACTGA 1501 GACCACGCTT TGATCCAGTT GGCCCACTTC CAGGACCTAA CCCCATCTTG CCAGGGCGAG 1561 GCGGCCCCAA TGACAGATTT CCCTTTAGAC CCAGCAGGGG TCGGCCAACT GATGGCCGGC 1621 TGTCATTCAT GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1681 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1741 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACT CCAACAGTGT CAACTCATGG 1801 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t