Construct: ORF TRCN0000471542
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017093.1_s317c1
- Derived from:
- ccsbBroadEn_06346
- DNA Barcode:
- TTACAGCTTGTACGCCGGGCGTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GZMA (3001)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471542
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3001 | GZMA | granzyme A | NM_006144.4 | 99.6% | 99.2% | 13T>A;362T>C;420C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 852
- ORF length:
- 786
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggtatgag gaactccaat agatttctgg catcctctct ctcagttgtc gtttctctcc 121 tgctaattcc tgaagatgtc tgtgaaaaaa ttattggagg aaatgaagta actcctcatt 181 caagacccta catggtccta cttagtcttg acagaaaaac catctgtgct ggggctttga 241 ttgcaaaaga ctgggtgttg actgcagctc actgtaactt gaacaaaagg tcccaggtca 301 ttcttggggc tcactcaata accagggaag agccaacaaa acagataatg cttGTTAAGA 361 AAGAGTTTCC CTATCCATGC TATGACCCAG CCACACGCGA AGGTGACCTT AAACTTTTAC 421 AGCTGACGGA AAAAGCAAAA ATTAACAAAT ATGTGACTAT CCTTCATCTA CCTAAAAAGG 481 GGGATGATGT GAAACCAGGA ACCATGTGCC AAGTTGCAGG GTGGGGCAGG ACTCACAATA 541 GTGCATCTTG GTCCGATACT CTGAGAGAAG TCAATATCAC CATCATAGAC AGAAAAGTCT 601 GCAATGATCG AAATCACTAT AATTTTAACC CTGTGATTGG AATGAATATG GTTTGTGCTG 661 GAAGCCTCCG AGGTGGAAGA GACTCGTGCA ATGGAGATTC TGGAAGCCCT TTGTTGTGCG 721 AGGGTGTTTT CCGAGGGGTC ACTTCCTTTG GCCTTGAAAA TAAATGCGGA GACCCTCGTG 781 GGCCTGGTGT CTATATTCTT CTCTCAAAGA AACACCTCAA CTGGATAATT ATGACTATCA 841 AGGGAGCAGT TTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 901 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 961 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTA CAGCTTGTAC GCCGGGCGTC 1021 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t