Construct: ORF TRCN0000471586
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015593.1_s317c1
- Derived from:
- ccsbBroadEn_12688
- DNA Barcode:
- GGCACCGCGCGAGCTTCCGGTAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIGZ (80235)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471586
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80235 | PIGZ | phosphatidylinositol glycan... | NM_025163.4 | 99.6% | 99.4% | (many diffs) |
| 2 | human | 80235 | PIGZ | phosphatidylinositol glycan... | XM_011513190.2 | 93.3% | 93.2% | (many diffs) |
| 3 | human | 80235 | PIGZ | phosphatidylinositol glycan... | XM_011513192.2 | 93.3% | 93.2% | (many diffs) |
| 4 | human | 80235 | PIGZ | phosphatidylinositol glycan... | XM_017007240.2 | 89.5% | 89.4% | (many diffs) |
| 5 | human | 80235 | PIGZ | phosphatidylinositol glycan... | XM_017007241.1 | 89.5% | 89.4% | (many diffs) |
| 6 | human | 80235 | PIGZ | phosphatidylinositol glycan... | XM_024453766.1 | 89.5% | 89.4% | (many diffs) |
| 7 | human | 80235 | PIGZ | phosphatidylinositol glycan... | XM_024453767.1 | 89.5% | 89.4% | (many diffs) |
| 8 | human | 80235 | PIGZ | phosphatidylinositol glycan... | XM_017007242.1 | 73.8% | 73.5% | (many diffs) |
| 9 | human | 80235 | PIGZ | phosphatidylinositol glycan... | XM_017007243.2 | 73.8% | 73.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1803
- ORF length:
- 1737
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gatctgtgga tccagcgtag catctgtagc agctgggaca tcattccagg 121 ttttgggccc ggtgtgttgg caacaactgg atctgaagat ggcagtcagg gtgctttggg 181 gtggtctcag cctgctccga gtgctgtggt gtctccttcc gcagacgggc tatgtgcacc 241 cagatgagtt cttccagtcc cctgaggtga tggcagagga catcctgggc gttcaggccg 301 cgcggccctg ggagttttac cccagcagct cctgccgctc ggtgctcttc cccctgctga 361 tctccggttc caccttctgg ctgctcaggc tctgggagga gctggggccg tggcctggcc 421 tggtgagcgg ctatgcgctg ctggtggggc ctcgactcct cctcactgcc ctttcctttg 481 ctctggacgg ggccgtgtac cacctggccc cgccgatggg ggcggatcgc tggaacgccc 541 tggccctgct gtctggttcc tacgtcaccc tggtcttcta cacaaggacc ttctccaaca 601 ccattgaggg actcctcttc acgtggctgc tggtgctggt atcctcccat gtaacgtggg 661 gccctacacg caaggagccg gcgccgggtc cacggtggcg cagctggctt cttggaggca 721 ttgtggctgc tggcttcttc aaccggccca cctttctggc ctttgctgtg gtccccctct 781 acctctgggg cactcgtgga gccacaaacc ctggtttgaa gtctctgacc cgggaggccc 841 tggtgctgct ccctggggcg accctcacag cagcggtgtt tgtggccacg gacagctggt 901 atttctccag ccccgctaca tccaggaacc ttgtcctgac acctgtcaac ttcctgcact 961 acaacctgaa tccccaaaac ctggcgagac atggcacgca cgcgcggctc actcacctgg 1021 cagtcaacgg cttcctgctc ttcggggtgc tgcatgccca ggccctgcag gctgcgtggc 1081 aacagctgca agtcggcctc caggcctctg cacaaatggg cctcctgagg gcactgggtg 1141 cccggagcct gctgtccagc cccaggtcct atctccttct cctctacttc atgcctctgg 1201 ccctgctatc tgcctttagc caccaggagg ctcggttcct gattcccctc ctggtccccc 1261 tggtcctgct ttgtagtcca cagacgcagc ctgtgccttg gaagggcact gtggtcctct 1321 tcaacgccct cggtgccctc ctcttcggct gcctgcatca ggggggcctg gtgcctggcc 1381 tggagtacct ggagcaggtg gtccatgccc ctgtgctccC AAGCACACCC ACCCACTACA 1441 CACTCCTCTT CACTCACACC TACATGCCCC CCCGGCACCT CCTACACCTC CCAGGCCTGG 1501 GGGCACCAGT GGAGGTGGTG GACATAGGGG GGACTGAGGA CTGGGCCCTG TGCCAAACCC 1561 TGAAAAGCTT CACCAGACAA CCAGCCTGCC AAGTGGCTGG TGGGCCATGG CTCTGCCGCC 1621 TCTTTGTGGT AACCCCTGGC ACCACCAGGC GTGCCGTGGA GAAGTGCAGC TTCCCCTTCA 1681 AGAATGAAAC ACTTTTATTT CCCCATCTGA CCCTGGAGGA TCCACCAGCC CTGTCCTCCT 1741 TGCTGAGTGG GGCTTGGAGG GACCACCTCA GTCTTCACAT TGTGGAGCTG GGGGAAGAAA 1801 CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1861 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1921 GGCTTTATAT ATCTTGTGGA AAGGACGAGG CACCGCGCGA GCTTCCGGTA ATACGCGTTA 1981 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt