Construct: ORF TRCN0000471599

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017312.1_s317c1
Derived from:
ccsbBroadEn_11912
DNA Barcode:
TACGTAAATATTTCTGGTATGGCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SEC61A1 (29927)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471599
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 29927 SEC61A1 SEC61 translocon alpha 1 su... NM_013336.4 88.8% 88.8% 1_159del
2 human 55176 SEC61A2 SEC61 translocon alpha 2 su... NM_018144.4 69.4% 83.1% (many diffs)
3 mouse 53421 Sec61a1 Sec61 alpha 1 subunit (S. c... NM_016906.4 80% 88.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1335
ORF length:
1269
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc ttcagattca gctgaccctt tctattggat gagagtgatt ctagcctcta
121acagaggcac attgatggag ctagggatct ctcctattgt cacgtctggc cttataatgc
181aactcttggc tggcgccaag ataattgaag ttggtgacac cccaaaagac cgagctctct
241tcaacggagc ccaaaagtta tttggcatga tcattactat cggccagtct atcgtgtatg
301tgatgaccgg gatgtatggg gacccttctg aaatgggtgc tggaatttgc ctgctaatca
361ccattcagct ctttgttgct ggcttaattg tcctactttt ggatgaactc ctgcaaaaag
421gatatggcct tggctctggt atttctctct tcattgcaac taacatctgt gaaaccatcg
481tatggaaggc attcagcccc actactgtca acactggccg aggaatggaa tttgaaggtg
541ctatcatcgc acttttccat ctgctggcca cacgcacaga caaggtccga gcccttcggg
601aggcgttcta ccgccagaat cttcccaacc tcatgaatct catcgccacc atctttgtct
661ttgcagtggt catctatttc cagggcttcc gagtggacct gccaatcaag tcggcccgct
721accgtggcca gtacaacacc tatcccatca agctcttcta tacgtccaac atccccatca
781tcctgcagtc tgccctggtg tccaaccttt atgtcatctc ccaaatgctc tcagctcgct
841tcagtggcaa cttgctggtc agccTGCTGG GCACCTGGTC GGACACGTCT TCTGGGGGCC
901CAGCACGTGC TTATCCAGTT GGTGGCCTTT GCTATTACCT GTCCCCTCCA GAATCTTTTG
961GCTCCGTGTT AGAAGACCCG GTCCATGCAG TTGTATACAT AGTGTTCATG CTGGGCTCCT
1021GTGCATTCTT CTCCAAAACG TGGATTGAGG TCTCAGGTTC CTCTGCCAAA GATGTTGCAA
1081AGCAGCTGAA GGAGCAGCAG ATGGTGATGA GAGGCCACCG AGAGACCTCC ATGGTCCATG
1141AACTCAACCG GTACATCCCC ACAGCCGCGG CCTTTGGTGG GCTGTGCATC GGGGCCCTCT
1201CGGTCCTGGC TGACTTCCTA GGCGCCATTG GGTCTGGAAC CGGGATCCTG CTCGCAGTCA
1261CAATCATCTA CCAGTACTTT GAGATCTTCG TTAAGGAGCA AAGCGAGGTT GGCAGCATGG
1321GGGCCCTGCT CTTCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
1381CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
1441TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TACGTAAATA TTTCTGGTAT
1501GGCCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.