Construct: ORF TRCN0000471599
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017312.1_s317c1
- Derived from:
- ccsbBroadEn_11912
- DNA Barcode:
- TACGTAAATATTTCTGGTATGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEC61A1 (29927)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471599
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29927 | SEC61A1 | SEC61 translocon alpha 1 su... | NM_013336.4 | 88.8% | 88.8% | 1_159del |
| 2 | human | 55176 | SEC61A2 | SEC61 translocon alpha 2 su... | NM_018144.4 | 69.4% | 83.1% | (many diffs) |
| 3 | mouse | 53421 | Sec61a1 | Sec61 alpha 1 subunit (S. c... | NM_016906.4 | 80% | 88.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1335
- ORF length:
- 1269
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ttcagattca gctgaccctt tctattggat gagagtgatt ctagcctcta 121 acagaggcac attgatggag ctagggatct ctcctattgt cacgtctggc cttataatgc 181 aactcttggc tggcgccaag ataattgaag ttggtgacac cccaaaagac cgagctctct 241 tcaacggagc ccaaaagtta tttggcatga tcattactat cggccagtct atcgtgtatg 301 tgatgaccgg gatgtatggg gacccttctg aaatgggtgc tggaatttgc ctgctaatca 361 ccattcagct ctttgttgct ggcttaattg tcctactttt ggatgaactc ctgcaaaaag 421 gatatggcct tggctctggt atttctctct tcattgcaac taacatctgt gaaaccatcg 481 tatggaaggc attcagcccc actactgtca acactggccg aggaatggaa tttgaaggtg 541 ctatcatcgc acttttccat ctgctggcca cacgcacaga caaggtccga gcccttcggg 601 aggcgttcta ccgccagaat cttcccaacc tcatgaatct catcgccacc atctttgtct 661 ttgcagtggt catctatttc cagggcttcc gagtggacct gccaatcaag tcggcccgct 721 accgtggcca gtacaacacc tatcccatca agctcttcta tacgtccaac atccccatca 781 tcctgcagtc tgccctggtg tccaaccttt atgtcatctc ccaaatgctc tcagctcgct 841 tcagtggcaa cttgctggtc agccTGCTGG GCACCTGGTC GGACACGTCT TCTGGGGGCC 901 CAGCACGTGC TTATCCAGTT GGTGGCCTTT GCTATTACCT GTCCCCTCCA GAATCTTTTG 961 GCTCCGTGTT AGAAGACCCG GTCCATGCAG TTGTATACAT AGTGTTCATG CTGGGCTCCT 1021 GTGCATTCTT CTCCAAAACG TGGATTGAGG TCTCAGGTTC CTCTGCCAAA GATGTTGCAA 1081 AGCAGCTGAA GGAGCAGCAG ATGGTGATGA GAGGCCACCG AGAGACCTCC ATGGTCCATG 1141 AACTCAACCG GTACATCCCC ACAGCCGCGG CCTTTGGTGG GCTGTGCATC GGGGCCCTCT 1201 CGGTCCTGGC TGACTTCCTA GGCGCCATTG GGTCTGGAAC CGGGATCCTG CTCGCAGTCA 1261 CAATCATCTA CCAGTACTTT GAGATCTTCG TTAAGGAGCA AAGCGAGGTT GGCAGCATGG 1321 GGGCCCTGCT CTTCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1381 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1441 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TACGTAAATA TTTCTGGTAT 1501 GGCCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt