Construct: ORF TRCN0000471622
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011779.3_s317c1
- Derived from:
- ccsbBroadEn_11324
- DNA Barcode:
- TCCTTCTTACCGCACCACTCCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS6KA4 (8986)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471622
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8986 | RPS6KA4 | ribosomal protein S6 kinase A4 | XM_005274380.1 | 70.1% | 66.8% | (many diffs) |
| 2 | human | 8986 | RPS6KA4 | ribosomal protein S6 kinase A4 | NM_001300802.2 | 68.3% | 68.3% | (many diffs) |
| 3 | human | 8986 | RPS6KA4 | ribosomal protein S6 kinase A4 | NM_001006944.2 | 68.2% | 68.2% | (many diffs) |
| 4 | human | 8986 | RPS6KA4 | ribosomal protein S6 kinase A4 | NM_003942.3 | 67.7% | 67.7% | (many diffs) |
| 5 | human | 8986 | RPS6KA4 | ribosomal protein S6 kinase A4 | NM_001318361.2 | 59.5% | 59.5% | (many diffs) |
| 6 | mouse | 56613 | Rps6ka4 | ribosomal protein S6 kinase... | XM_017318262.1 | 63.5% | 51.5% | (many diffs) |
| 7 | mouse | 56613 | Rps6ka4 | ribosomal protein S6 kinase... | NM_019924.2 | 60% | 65.2% | (many diffs) |
| 8 | mouse | 56613 | Rps6ka4 | ribosomal protein S6 kinase... | XM_017318261.1 | 52.6% | 57.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1638
- ORF length:
- 1572
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ggacgaggac gacgatgaga gctgcgccgt ggagctgcgg atcacagaag 121 ccaacctgac cgggcacgag gagaaggtga gcgtggagaa cttcgagctg ctcaaggtgc 181 tgggcacggg agcctacggc aaggtgttcc tggtgcggaa ggcgggcggg cacgacgcgg 241 ggaagctgta cgccatgaag gtgctgcgca aggcggcgct ggtgcagcgc gccaagacgc 301 aggagcacac gcgcaccgag cgctcggtgc tggagctggt gcgccaggcg cccttcctgg 361 tcacgctgca ctacgctttc cagacggatg ccaagctgca cctcatcctg gactatgtga 421 gcggcgggga gatgttcacc cacctctacc agcgccagta cttcaaggag gctgaggtgc 481 gcgtgtatgg gggtgagatc gtgctggccc tggaacacct gcacaagctc ggcatcattt 541 accgagacct gaaactggag aatgtgctgc tggactccga gggccacatt gtcctcacgg 601 acttcgggct gagcaaggag ttcctgacgg aggagaaaga gcggaccttc tccttctgtg 661 gcaccatcga gtacatggcc cccgaaatca tccgtagcaa gacggggcat ggcaaggctg 721 tggactggtg gagcctgggc atcttgctct tcgagctgct gacgggggcc tcgcccttca 781 ccctggaggg cgagaggaac acgcaggctg aggtgtctcg acggatcctg aagtgctccc 841 ctcccttccc ccctcggatc gggcccgtgg cgcaggacct gctgcagcgg ctgctttgta 901 aggatcctaa gaagcgattg ggcgcggggc cccagggggc acaagaagtc cggaaccatc 961 ccttcttcca gggcctcgat tgggtggctc tggctgccag gaagattcca gccccattcc 1021 ggccccaaat ccgctcagag ctggatgtgg gcaactttgc ggaggaattc actcggctgg 1081 agcctgtcta ctcaccccct ggcagccccc cacctgggga cccccgaatc tttcagggat 1141 actcctttgt ggcaccctcc attctctttg accacaacaa cgcggccgag atcatgtgca 1201 aaatccgcga ggggcgcttc tcccttgacg ggGAGGCCTG GCAGGGTGTA TCCGAGGAAG 1261 CCAAGGAGCT GGTCCGAGGG CTCCTGACCG TGGACCCCGC CAAGCGGCTG AAGCTCGAGG 1321 GACTGCGGGG CAGCTCGTGG CTGCAGGACG GCAGCGCGCG CTCCTCGCCC CCGCTCCGGA 1381 CGCCCGACGT GCTCGAGTCC TCTGGGCCCG CAGTGCGCTC GGGTCTCAAC GCCACCTTCA 1441 TGGCATTCAA CCGGGGCAAG CGGGAGGGCT TCTTCCTGAA GAGCGTGGAG AACGCACCCC 1501 TGGCCAAGCG GCGGAAGCAG AAGCTGCGGA GCGCCACCGC CTCCCGCCGG GGCTCCCCTG 1561 CACCAGCCAA CCCGGGCCGA GCCCCCGTCG CCGCCAAAGG GGCCCCCCGC CGAGCCAACG 1621 GCCCCCTGCC CCCCTCCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1681 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1741 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCCTTCT TACCGCACCA 1801 CTCCGACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt