Construct: ORF TRCN0000471633
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001822.1_s317c1
- Derived from:
- ccsbBroadEn_01557
- DNA Barcode:
- AAGATACACTGCTCCTTTAGTTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMARCD1 (6602)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471633
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6602 | SMARCD1 | SWI/SNF related, matrix ass... | NM_003076.5 | 100% | 100% | |
| 2 | human | 6602 | SMARCD1 | SWI/SNF related, matrix ass... | NM_139071.3 | 92% | 92% | 1265_1266ins123 |
| 3 | human | 6602 | SMARCD1 | SWI/SNF related, matrix ass... | XM_005269107.4 | 92% | 92% | 531_532ins123 |
| 4 | human | 6602 | SMARCD1 | SWI/SNF related, matrix ass... | XR_944684.2 | 53.2% | 1_134del;1680_2901del | |
| 5 | human | 6602 | SMARCD1 | SWI/SNF related, matrix ass... | XM_011538695.2 | 50.3% | 50% | (many diffs) |
| 6 | human | 6602 | SMARCD1 | SWI/SNF related, matrix ass... | XR_944683.2 | 47.7% | 1_134del;1680_3236del | |
| 7 | mouse | 83797 | Smarcd1 | SWI/SNF related, matrix ass... | NM_031842.2 | 93.2% | 99.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1611
- ORF length:
- 1545
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcccgggcg ggtttccagt ctgtggctcc aagcggcggc gccggagcct 121 caggaggggc gggcgcggct gctgccttgg gcccgggcgg aactccgggg cctcctgtgc 181 gaatgggccc ggctccgggt caagggctgt accgctcccc gatgcccgga gcggcctatc 241 cgagaccagg tatgttgcca ggcagccgaa tgacacctca gggaccttcc atgggacccc 301 ctggctatgg ggggaaccct tcagtccgac ctggcctggc ccagtcaggg atggatcagt 361 cccgcaagag acctgcccct cagcagatcc agcaggtcca gcagcaggcg gtccaaaatc 421 gaaaccacaa tgcaaagaaa aagaagatgg ctgacaaaat tctacctcaa aggattcgtg 481 aactggtacc agaatcccag gcctatatgg atctcttggc ttttgaaagg aaactggacc 541 agactatcat gaggaaacgg ctagatatcc aagaggcctt gaaacgtccc atcaagcaaa 601 aacggaagct gcgaattttc atttctaaca ctttcaatcc ggctaagtca gatgccgagg 661 atggggaagg gacggtggct tcctgggagc ttcgggtaga aggacggctc ctggaggatt 721 cagccttgtc caaatatgat gccactaaac aaaagaggaa gttctcttcc ttttttaagt 781 ccttggtgat tgaactggac aaagacctgt atgggccaga caaccatctg gtagaatggc 841 acaggaccgc cactacccag gagaccgatg gctttcaggt gaagcggccg ggagacgtga 901 atgtacggtg tactgtccta ctgatgctgg attaccagcc tccccagttt aaattagacc 961 cccgcctagc tcgactcctg ggcatccata cccagactcg tccagtgatc atccaagcac 1021 tgtggcaata tattaagaca cataagctcc aggaccctca cgagcgggag tttgtcatct 1081 gtgacaagta cctgcagcag atctttgagt ctcaacgtat gaagttttca gagatcccTC 1141 AGCGGCTCCA TGCCTTGCTT ATGCCACCAG AACCTATCAT CATTAATCAT GTCATCAGTG 1201 TTGACCCGAA TGATCAGAAA AAGACAGCTT GTTATGACAT TGATGTTGAA GTGGATGACA 1261 CCTTGAAGAC CCAGATGAAT TCTTTTCTGC TGTCCACTGC CAGCCAACAG GAGATTGCTA 1321 CTCTAGACAA CAAGATCCAT GAGACAATAG AAACCATCAA CCAGCTGAAG ACTCAGCGGG 1381 AGTTCATGCT GAGCTTTGCC AGAGACCCTC AGGGTTTCAT CAATGACTGG CTTCAGTCCC 1441 AGTGCAGGGA CCTCAAGACA ATGACTGATG TGGTGGGTAA CCCAGAGGAG GAGCGCCGAG 1501 CTGAGTTCTA CTTCCAGCCC TGGGCTCAGG AGGCTGTGTG CCGATACTTC TACTCCAAGG 1561 TGCAGCAGAG ACGACAAGAA TTAGAGCAAG CCCTGGGAAT CCGGAATACA TACCCAACTT 1621 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1681 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1741 CTTGTGGAAA GGACGAAAGA TACACTGCTC CTTTAGTTCA ACGCGTTAAG TCgacaatca 1801 acctctggat tacaaaattt gtgaaagatt