Construct: ORF TRCN0000471634
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005270.1_s317c1
- Derived from:
- ccsbBroadEn_05330
- DNA Barcode:
- GGTAGGAACTCCTGGCAATTTATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP4X1 (260293)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471634
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 260293 | CYP4X1 | cytochrome P450 family 4 su... | NM_178033.2 | 100% | 100% | |
| 2 | human | 260293 | CYP4X1 | cytochrome P450 family 4 su... | XM_017000973.2 | 92.2% | 91.5% | (many diffs) |
| 3 | human | 260293 | CYP4X1 | cytochrome P450 family 4 su... | NM_001320289.1 | 90.9% | 87.8% | (many diffs) |
| 4 | human | 260293 | CYP4X1 | cytochrome P450 family 4 su... | NM_001320290.2 | 87.2% | 87.2% | 0_1ins195 |
| 5 | human | 260293 | CYP4X1 | cytochrome P450 family 4 su... | XR_001737105.2 | 63.3% | 1_243del;1277_1278ins38;1733_2312del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1593
- ORF length:
- 1527
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga attctcctgg ctggagacgc gctgggcgcg gcccttttac ctggcgttcg 121 tgttctgcct ggccctgggg ctgctgcagg ccattaagct gtacctgcgg aggcagcggc 181 tgctgcggga cctgcgcccc ttcccagcgc cccccaccca ctggttcctt gggcaccaga 241 agtttattca ggatgataac atggagaagc ttgaggaaat tattgaaaaa taccctcgtg 301 ccttcccttt ctggattggg ccctttcagg catttttctg tatctatgac ccagactatg 361 caaagacact tctgagcaga acagatccca agtcccagta cctgcagaaa ttctcacctc 421 cacttcttgg aaaaggacta gcggctctag acggacccaa gtggttccag catcgtcgcc 481 tactaactcc tggattccat tttaacatcc tgaaagcata cattgaggtg atggctcatt 541 ctgtgaaaat gatgctggat aagtgggaga agatttgcag cactcaggac acaagcgtgg 601 aggtctatga gcacatcaac tcgatgtctc tggatataat catgaaatgc gctttcagca 661 aggagaccaa ctgccagaca aacagcaccc atgatcctta tgcaaaagcc atatttgaac 721 tcagcaaaat catatttcac cgcttgtaca gtttgttgta tcacagtgac ataattttca 781 aactcagccc tcagggctac cgcttccaga agttaagccg agtgttgaat cagtacacag 841 atacaataat ccaggaaaga aagaaatccc tccaggctgg ggtaaagcag gataacactc 901 cgaagaggaa gtaccaggat tttctggata ttgtcctttc tgccaaggat gaaagtggta 961 gcagcttctc agatattgat gtacactctg aagtgagcac attcctgttg gcaggacatg 1021 acaccttggc agcaagcatc tcctggatcc tttactgcct ggctctgaac cctgagcatc 1081 aagagagatg ccgggaggag gtcaggggca tcctggggga tgggTCTTCT ATCACTTGGG 1141 ACCAGCTGGG TGAGATGTCG TACACCACAA TGTGCATCAA GGAGACGTGC CGATTGATTC 1201 CTGCAGTCCC GTCCATTTCC AGAGATCTCA GCAAGCCACT TACCTTCCCA GATGGATGCA 1261 CATTGCCTGC AGGGATCACC GTGGTTCTTA GTATTTGGGG TCTTCACCAC AACCCTGCTG 1321 TCTGGAAAAA CCCAAAGGTC TTTGACCCCT TGAGGTTCTC TCAGGAGAAT TCTGATCAGA 1381 GACACCCCTA TGCCTACTTA CCATTCTCAG CTGGATCAAG GAACTGCATT GGGCAGGAGT 1441 TTGCCATGAT TGAGTTAAAG GTAACCATTG CCTTGATTCT GCTCCACTTC AGAGTGACTC 1501 CAGACCCCAC CAGGCCTCTT ACTTTCCCCA ACCATTTTAT CCTCAAGCCC AAGAATGGGA 1561 TGTATTTGCA CCTGAAGAAA CTCTCTGAAT GTTACCCAAC TTTCTTGTAC AAAGTGGTTG 1621 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1681 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGG 1741 TAGGAACTCC TGGCAATTTA TAACGCGTTA AGTCgacaat caacctctgg attacaaaat 1801 ttgtgaaaga tt