Construct: ORF TRCN0000471635
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008376.1_s317c1
- Derived from:
- ccsbBroadEn_04242
- DNA Barcode:
- TGTTACTGCCAAGACTACCCAAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC130 (81576)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471635
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81576 | CCDC130 | coiled-coil domain containi... | NM_001320561.2 | 100% | 100% | |
| 2 | human | 81576 | CCDC130 | coiled-coil domain containi... | NM_001320564.2 | 100% | 100% | |
| 3 | human | 81576 | CCDC130 | coiled-coil domain containi... | NM_001320565.2 | 100% | 100% | |
| 4 | human | 81576 | CCDC130 | coiled-coil domain containi... | NM_030818.4 | 100% | 100% | |
| 5 | human | 81576 | CCDC130 | coiled-coil domain containi... | XM_005260086.4 | 100% | 100% | |
| 6 | human | 81576 | CCDC130 | coiled-coil domain containi... | NM_001320569.2 | 87.6% | 87.6% | 0_1ins147 |
| 7 | human | 81576 | CCDC130 | coiled-coil domain containi... | XR_001753768.2 | 68.1% | 1_250del;823_900del;1517_1743del | |
| 8 | human | 81576 | CCDC130 | coiled-coil domain containi... | NM_001320566.2 | 63.3% | 63.3% | 0_1ins435 |
| 9 | human | 81576 | CCDC130 | coiled-coil domain containi... | NM_001320567.2 | 63.3% | 63.3% | 0_1ins435 |
| 10 | human | 81576 | CCDC130 | coiled-coil domain containi... | NM_001320568.2 | 63.3% | 63.3% | 0_1ins435 |
| 11 | human | 81576 | CCDC130 | coiled-coil domain containi... | XM_011528326.2 | 51.4% | 46.6% | (many diffs) |
| 12 | mouse | 67736 | Ccdc130 | coiled-coil domain containi... | NM_001294281.1 | 43.8% | 46.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1254
- ORF length:
- 1188
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg tgaaaggaaa ggggtcaaca agtactatcc tccagacttc aaccctgaga 121 agcatggctc tctcaaccga taccacaaca gccacccgct tcgggagcgg gctcggaagc 181 tgtcacaggg catcctcatc atccgattcg aaatgccata taacatctgg tgcgatggct 241 gcaagaacca catcggcatg ggtgttcgtt acaatgcaga aaagaagaag gtgggcaatt 301 actacacaac cccgatctac aggttccgga tgaaatgcca cctctgtgtc aactacatcg 361 agatgcagac ggaccccgcc aactgcgact acgtgatcgt gagtggcgcc cagcgcaagg 421 aggagcgctg ggacatggcg gacaatgagc aggtgctgac cacagagcat gagaagaagc 481 agaagctgga gacggacgcc atgttccggc tggagcatgg cgaggccgac cgcagcacac 541 tcaagaaggc gctgcccaca ctgagccaca tccaggaggc ccagagcgcc tggaaggacg 601 acttcgccct caacagcatg ctgcggagaa ggttccggga aaagaaaaaa gccatccagg 661 aggaggagga gagagaccag gccttgcagg ccaaggcgag cctgaccatc ccgctggtgc 721 ccgagacgga agatgaccgc aagctggcgg ctctgctgaa gttccacacc ctggactccT 781 ACGAGGACAA GCAGAAACTC AAGCGGACCG AGATCATCAG CCGCTCCTGG TTCCCCTCTG 841 CCCCCGGATC CGCCTCCAGC AGCAAGGTCA GCGGCGTCCT GAAGAAGCTG GCACAGAGCC 901 GCAGAACCGC GCTTGCCACC TCCCCCATCA CCGTCGGGGA CCTGGGCATC GTGCGGCGGA 961 GGTCTCGGGA CGTCCCGGAG AGCCCCCAGC ATGCGGCCGA CACCCCCAAG TCTGGGGAAC 1021 CGCGGGTACC AGAGGAGGCT GCCCAGGACC GGCCCATGTC CCCCGGAGAC TGTCCTCCGG 1081 AAACAACTGA GACCCCCAAG TGCAGCAGCC CGAGGGGGCA GGAAGGGAGC CGTCAGGACA 1141 AGCCCCTGTC GCCAGCAGGC TCCTCCCAGG AGGCAGCTGA CACCCCCGAC ACGCGGCACC 1201 CCTGCAGTCT CGGCTCCTCC CTCGTGGCGG ACTACTCCGA CTCGGAGAGT GAGTGCCCGA 1261 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1321 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1381 TATCTTGTGG AAAGGACGAT GTTACTGCCA AGACTACCCA AGGACGCGTT AAGTCgacaa 1441 tcaacctctg gattacaaaa tttgtgaaag att