Construct: ORF TRCN0000471640

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007236.1_s317c1
Derived from:
ccsbBroadEn_08152
DNA Barcode:
ACAGAACGGACCTCTGCTCTAATC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PSAT1 (29968)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471640
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 29968 PSAT1 phosphoserine aminotransfer... NM_058179.4 99.9% 100% 297T>G
2 human 29968 PSAT1 phosphoserine aminotransfer... NM_021154.5 87.4% 87.5% 297T>G;869_870ins138
3 mouse 107272 Psat1 phosphoserine aminotransfer... NM_177420.2 86.2% 92.4% (many diffs)
4 mouse 107272 Psat1 phosphoserine aminotransfer... NM_001205339.1 83.8% 85.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1176
ORF length:
1110
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga cgcccccagg caggtggtca actttgggcc tggtcccgcc aagctgccgc
121actcagtgtt gttagagata caaaaggaat tattagacta caaaggagtt ggcattagtg
181ttcttgaaat gagtcacagg tcatcagatt ttgccaagat tattaacaat acagagaatc
241ttgtgcggga attgctagct gttccagaca actataaggt gatttttctg caaggaggtg
301ggtgcggcca gttcagtgct gtccccttaa acctcattgg cttgaaagca ggaaggtgtg
361cggactatgt ggtgacagga gcttggtcag ctaaggccgc agaagaagcc aagaagtttg
421ggactataaa tatcgttcac cctaaacttg ggagttatac aaaaattcca gatccaagca
481cctggaacct caacccagat gcctcctacg tgtattattg cgcaaatgag acggtgcatg
541gtgtggagtt tgactttata cccgatgtca agggagcagt actggtttgt gacatgtcct
601caaacttcct gtccaagcca gtggatgttt ccaagtttgg tgtgattttt gctggtgccc
661agaagaatgt tggcTCTGCT GGGGTCACCG TGGTGATTGT CCGTGATGAC CTGCTGGGGT
721TTGCCCTCCG AGAGTGCCCC TCGGTCCTGG AATACAAGGT GCAGGCTGGA AACAGCTCCT
781TGTACAACAC GCCTCCATGT TTCAGCATCT ACGTCATGGG CTTGGTTCTG GAGTGGATTA
841AAAACAATGG AGGTGCCGCG GCCATGGAGA AGCTTAGCTC CATCAAATCT CAAACAATTT
901ATGAGATTAT TGATAATTCT CAAGGATTCT ACGTTTGTCC AGTGGAGCCC CAAAATAGAA
961GCAAGATGAA TATTCCATTC CGCATTGGCA ATGCCAAAGG AGATGATGCT TTAGAAAAAA
1021GATTTCTTGA TAAAGCTCTT GAACTCAATA TGTTGTCCTT GAAAGGGCAT AGGTCTGTGG
1081GAGGCATCCG GGCCTCTCTG TATAATGCTG TCACAATTGA AGACGTTCAG AAGCTGGCCG
1141CCTTCATGAA AAAATTTTTG GAGATGCATC AGCTATGCCC AACTTTCTTG TACAAAGTGG
1201TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1261CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1321AACAGAACGG ACCTCTGCTC TAATCACGCG TTAAGTCgac aatcaacctc tggattacaa
1381aatttgtgaa agatt

Download FASTA (ORF) (Full)