Construct: ORF TRCN0000471640
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007236.1_s317c1
- Derived from:
- ccsbBroadEn_08152
- DNA Barcode:
- ACAGAACGGACCTCTGCTCTAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSAT1 (29968)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471640
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29968 | PSAT1 | phosphoserine aminotransfer... | NM_058179.4 | 99.9% | 100% | 297T>G |
| 2 | human | 29968 | PSAT1 | phosphoserine aminotransfer... | NM_021154.5 | 87.4% | 87.5% | 297T>G;869_870ins138 |
| 3 | mouse | 107272 | Psat1 | phosphoserine aminotransfer... | NM_177420.2 | 86.2% | 92.4% | (many diffs) |
| 4 | mouse | 107272 | Psat1 | phosphoserine aminotransfer... | NM_001205339.1 | 83.8% | 85.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1176
- ORF length:
- 1110
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgcccccagg caggtggtca actttgggcc tggtcccgcc aagctgccgc 121 actcagtgtt gttagagata caaaaggaat tattagacta caaaggagtt ggcattagtg 181 ttcttgaaat gagtcacagg tcatcagatt ttgccaagat tattaacaat acagagaatc 241 ttgtgcggga attgctagct gttccagaca actataaggt gatttttctg caaggaggtg 301 ggtgcggcca gttcagtgct gtccccttaa acctcattgg cttgaaagca ggaaggtgtg 361 cggactatgt ggtgacagga gcttggtcag ctaaggccgc agaagaagcc aagaagtttg 421 ggactataaa tatcgttcac cctaaacttg ggagttatac aaaaattcca gatccaagca 481 cctggaacct caacccagat gcctcctacg tgtattattg cgcaaatgag acggtgcatg 541 gtgtggagtt tgactttata cccgatgtca agggagcagt actggtttgt gacatgtcct 601 caaacttcct gtccaagcca gtggatgttt ccaagtttgg tgtgattttt gctggtgccc 661 agaagaatgt tggcTCTGCT GGGGTCACCG TGGTGATTGT CCGTGATGAC CTGCTGGGGT 721 TTGCCCTCCG AGAGTGCCCC TCGGTCCTGG AATACAAGGT GCAGGCTGGA AACAGCTCCT 781 TGTACAACAC GCCTCCATGT TTCAGCATCT ACGTCATGGG CTTGGTTCTG GAGTGGATTA 841 AAAACAATGG AGGTGCCGCG GCCATGGAGA AGCTTAGCTC CATCAAATCT CAAACAATTT 901 ATGAGATTAT TGATAATTCT CAAGGATTCT ACGTTTGTCC AGTGGAGCCC CAAAATAGAA 961 GCAAGATGAA TATTCCATTC CGCATTGGCA ATGCCAAAGG AGATGATGCT TTAGAAAAAA 1021 GATTTCTTGA TAAAGCTCTT GAACTCAATA TGTTGTCCTT GAAAGGGCAT AGGTCTGTGG 1081 GAGGCATCCG GGCCTCTCTG TATAATGCTG TCACAATTGA AGACGTTCAG AAGCTGGCCG 1141 CCTTCATGAA AAAATTTTTG GAGATGCATC AGCTATGCCC AACTTTCTTG TACAAAGTGG 1201 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1261 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1321 AACAGAACGG ACCTCTGCTC TAATCACGCG TTAAGTCgac aatcaacctc tggattacaa 1381 aatttgtgaa agatt