Construct: ORF TRCN0000471658
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018794.3_s317c1
- Derived from:
- ccsbBroadEn_15195
- DNA Barcode:
- TCGCAGGTTCGGGTATGCACGCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NTPCR (84284)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471658
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84284 | NTPCR | nucleoside-triphosphatase, ... | NM_032324.3 | 100% | 100% | |
| 2 | human | 84284 | NTPCR | nucleoside-triphosphatase, ... | NM_001329453.2 | 63.1% | 63.1% | 291_292ins210 |
| 3 | human | 84284 | NTPCR | nucleoside-triphosphatase, ... | NM_001329452.2 | 47.4% | 46.2% | 295_296ins43;298_299ins163;365_561del |
| 4 | human | 84284 | NTPCR | nucleoside-triphosphatase, ... | NR_138027.1 | 39.2% | (many diffs) | |
| 5 | human | 84284 | NTPCR | nucleoside-triphosphatase, ... | NR_138026.2 | 6.4% | 1_100del;133_134ins163;508_6161del | |
| 6 | human | 84284 | NTPCR | nucleoside-triphosphatase, ... | NR_138024.2 | 6.4% | (many diffs) | |
| 7 | human | 84284 | NTPCR | nucleoside-triphosphatase, ... | NR_138025.2 | 5.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 639
- ORF length:
- 570
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcccggcac gtgttcctaa cggggccccc aggagttgga aaaacaacat 121 tgatccataa agccagtgag gttttaaaat cctctggtgt gcctgttgat ggattttata 181 ccgaagaagt cagacaggga gggagaagaa taggattcga tgtcgtcacg ttgtccggca 241 cccgggggCC TTTATCGAGA GTTGGGTTAG AGCCTCCACC TGGAAAACGT GAATGCCGAG 301 TTGGGCAGTA TGTGGTCGAC CTGACTTCTT TTGAGCAGTT GGCACTACCC GTCTTGAGGA 361 ATGCCGACTG CAGCAGTGGC CCAGGGCAAA GAGTGTGCGT CATCGATGAG ATTGGGAAGA 421 TGGAGCTCTT CAGTCAGCTT TTCATTCAAG CTGTTCGTCA GACGCTGTCT ACCCCAGGGA 481 CTATAATCCT TGGCACAATC CCAGTTCCTA AAGGAAAGCC ACTGGCTCTT GTAGAAGAAA 541 TCAGAAACAG AAAGGATGTG AAGGTGTTTA ATGTCACCAA GGAAAACAGA AACCACCTTC 601 TGCCAGATAT CGTGACGTGC GTGCAGAGCA GCAGGAAGTT GCCAACTTTC TTGTACAAAG 661 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 721 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 781 ACGATCGCAG GTTCGGGTAT GCACGCTTAC GCGTTAAGTC gacaatcaac ctctggatta 841 caaaatttgt gaaagatt