Construct: ORF TRCN0000471661
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012480.1_s317c1
- Derived from:
- ccsbBroadEn_00830
- DNA Barcode:
- ACTACTTTTTTCTTTATTTCTCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFNA21 (3452)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471661
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3452 | IFNA21 | interferon alpha 21 | NM_002175.2 | 100% | 100% | |
| 2 | human | 3441 | IFNA4 | interferon alpha 4 | NM_021068.2 | 95.9% | 92% | (many diffs) |
| 3 | human | 3451 | IFNA17 | interferon alpha 17 | NM_021268.2 | 95.5% | 91% | (many diffs) |
| 4 | human | 3446 | IFNA10 | interferon alpha 10 | NM_002171.2 | 95.2% | 91% | (many diffs) |
| 5 | human | 3444 | IFNA7 | interferon alpha 7 | NM_021057.2 | 94% | 87.8% | (many diffs) |
| 6 | human | 3449 | IFNA16 | interferon alpha 16 | NM_002173.3 | 92.9% | 85.7% | (many diffs) |
| 7 | human | 3442 | IFNA5 | interferon alpha 5 | NM_002169.2 | 91.5% | 86.7% | (many diffs) |
| 8 | human | 3448 | IFNA14 | interferon alpha 14 | NM_002172.3 | 90.6% | 82% | (many diffs) |
| 9 | human | 3445 | IFNA8 | interferon alpha 8 | NM_002170.4 | 89.7% | 81.4% | (many diffs) |
| 10 | human | 3443 | IFNA6 | interferon alpha 6 | NM_021002.2 | 89.6% | 81.4% | (many diffs) |
| 11 | human | 3439 | IFNA1 | interferon alpha 1 | NM_024013.3 | 89.4% | 80.9% | (many diffs) |
| 12 | human | 3440 | IFNA2 | interferon alpha 2 | NM_000605.4 | 89.2% | 81.4% | (many diffs) |
| 13 | human | 3447 | IFNA13 | interferon alpha 13 | NM_006900.4 | 89.1% | 81% | (many diffs) |
| 14 | human | 3453 | IFNA22P | interferon alpha 22, pseudo... | NR_036676.1 | 48.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 633
- ORF length:
- 567
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cctgtccttt tctttactga tggccgtgct ggtgctcagc tacaaatcca 121 tctgttctct gggctgtgat ctgcctcaga cccacagcct gggtaatagg agggccttga 181 tactcctggc acaaatggga agaatctctc ctttctcctg ccTGAAGGAC AGACATGACT 241 TTGGATTCCC CCAGGAGGAG TTTGATGGCA ACCAGTTCCA GAAGGCTCAA GCCATCTCTG 301 TCCTCCATGA GATGATCCAG CAGACCTTCA ATCTCTTCAG CACAAAGGAC TCATCTGCTA 361 CTTGGGAACA GAGCCTCCTA GAAAAATTTT CCACTGAACT TAACCAGCAG CTGAATGACC 421 TGGAAGCCTG CGTGATACAG GAGGTTGGGG TGGAAGAGAC TCCCCTGATG AATGTGGACT 481 CCATCCTGGC TGTGAAGAAA TACTTCCAAA GAATCACTCT TTATCTGACA GAGAAGAAAT 541 ACAGCCCTTG TGCCTGGGAG GTTGTCAGAG CAGAAATCAT GAGATCCTTC TCTTTATCAA 601 AAATTTTTCA AGAAAGATTA AGGAGGAAGG AATGCCCAAC TTTCTTGTAC AAAGTGGTTG 661 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 721 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC 781 TACTTTTTTC TTTATTTCTC CAACGCGTTA AGTCgacaat caacctctgg attacaaaat 841 ttgtgaaaga tt