Construct: ORF TRCN0000471662
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007752.1_s317c1
- Derived from:
- ccsbBroadEn_07489
- DNA Barcode:
- ACTAGGATCAGATTAAGATCTACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL19 (9801)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471662
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9801 | MRPL19 | mitochondrial ribosomal pro... | NM_014763.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 942
- ORF length:
- 876
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcctgcatt gcagcggggc actgggctgc aatgggccta ggccggagtt 121 tccaagccgc caggactctg ctccccccgc cggcctctat cgcctgcagg gtccacgcgg 181 ggcctgtccg gcagcagagc actgggcctt ccgagcccgg tgcgttccaa ccgccgccga 241 aaccggtcat cgtggacaag caccgccccg tggaaccgga acgcaggttc ttgagtcctg 301 aattcattcc tcgaagggga agaacagatc ctctgaaatt tcaaatagaa agaaaagata 361 tgttagaaag gagaaaagta ctccacattc cagagttcta tgttggaagt attcttcgtg 421 ttactacagc tgacccatat gccagtggaa aaatcagcca gtttctgggg atttgcattc 481 agagatcagg aagaggactt ggagctactt tcatccttag gaatgttatc gaaggacaag 541 gtgtcgagat ttgctttgaa ctttataatc cTCGGGTCCA GGAGATTCAG GTGGTCAAAT 601 TAGAGAAACG GCTGGATGAT AGCTTGCTAT ACTTACGAGA TGCCCTTCCT GAATATAGCA 661 CTTTTGATGT GAATATGAAG CCAGTAGTAC AAGAGCCTAA CCAAAAAGTT CCTGTTAATG 721 AGCTGAAAGT AAAAATGAAG CCTAAGCCCT GGTCTAAACG CTGGGAACGT CCAAATTTTA 781 ATATTAAAGG AATCAGATTT GATCTTTGTT TAACTGAACA GCAAATGAAA GAAGCTCAGA 841 AGTGGAATCA GCCATGGCTT GAATTTGATA TGATGAGGGA ATATGATACT TCAAAAATTG 901 AAGCTGCAAT ATGGAAGGAA ATTGAAGCGT CGAAAAGGTC TTGCCCAACT TTCTTGTACA 961 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1021 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1081 AGGACGAACT AGGATCAGAT TAAGATCTAC GACGCGTTAA GTCgacaatc aacctctgga 1141 ttacaaaatt tgtgaaagat t