Construct: ORF TRCN0000471686
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006845.1_s317c1
- Derived from:
- ccsbBroadEn_00245
- DNA Barcode:
- TGTTGCATCCACGTCATTGGGAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CD3G (917)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471686
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 917 | CD3G | CD3g molecule | NM_000073.2 | 100% | 100% | |
| 2 | human | 917 | CD3G | CD3g molecule | XM_006718941.3 | 100% | 100% | |
| 3 | human | 917 | CD3G | CD3g molecule | XM_005271724.4 | 84.9% | 76.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 615
- ORF length:
- 546
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaacagggg aagggcctgg ctgtccTCAT CCTGGCTATC ATTCTTCTTC 121 AAGGTACTTT GGCCCAGTCA ATCAAAGGAA ACCACTTGGT TAAGGTGTAT GACTATCAAG 181 AAGATGGTTC GGTACTTCTG ACTTGTGATG CAGAAGCCAA AAATATCACA TGGTTTAAAG 241 ATGGGAAGAT GATCGGCTTC CTAACTGAAG ATAAAAAAAA ATGGAATCTG GGAAGTAATG 301 CCAAGGACCC TCGAGGGATG TATCAGTGTA AAGGATCACA GAACAAGTCA AAACCACTCC 361 AAGTGTATTA CAGAATGTGT CAGAACTGCA TTGAACTAAA TGCAGCCACC ATATCTGGCT 421 TTCTCTTTGC TGAAATCGTC AGCATTTTCG TCCTTGCTGT TGGGGTCTAC TTCATTGCTG 481 GACAGGATGG AGTTCGCCAG TCGAGAGCTT CAGACAAGCA GACTCTGTTG CCCAATGACC 541 AGCTCTACCA GCCCCTCAAG GATCGAGAAG ATGACCAGTA CAGCCACCTT CAAGGAAACC 601 AGTTGAGGAG GAATTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 661 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 721 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TGTTGCATCC ACGTCATTGG 781 GAAGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt