Construct: ORF TRCN0000471692
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012651.1_s317c1
- Derived from:
- ccsbBroadEn_03841
- DNA Barcode:
- CCCACTTCGCTGCCACTTATGATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIAA1586 (57691)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471692
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57691 | KIAA1586 | KIAA1586 | NM_020931.4 | 100% | 100% | |
| 2 | human | 57691 | KIAA1586 | KIAA1586 | NM_001286274.1 | 96.5% | 96.5% | 104_105ins81 |
| 3 | human | 57691 | KIAA1586 | KIAA1586 | NM_001286275.1 | 91.4% | 91.4% | 0_1ins201 |
| 4 | human | 57691 | KIAA1586 | KIAA1586 | NM_001286276.1 | 91.4% | 91.4% | 0_1ins201 |
| 5 | human | 57691 | KIAA1586 | KIAA1586 | XM_011514767.2 | 19.9% | 19.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2427
- ORF length:
- 2361
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg agacccgggg tcggaaataa tagaatctgt ccctccagct ggccctgagg 121 catctgagtc aacaacggat gaaaatgaag acgacattca gtttgtcagt gaaggaccat 181 cgagacctgt tcttgaatac atcgatctgg tctgtggtga tgatgaaaac cctagcgcct 241 attatagtga tattctgttt cctaaaatgc caaaacgaca gggtgatttt ttgcattttt 301 taaatgtgaa gaaggtgaaa acagacacag aaaataatga agtgagcaaa aatcactgca 361 gattgtctaa ggcaaaggaa ccacatttcg agtatattga acaaccaatc attgaagaaa 421 agccatcact ttcatcaaag aaagaaatag ataatcttgt gcttccagat tgttggaatg 481 aaaaacaagc atttatgttt acagaacaat acaaatggct tgaaataaaa gaaggtaaat 541 taggatgtaa ggattgttca gcagttcggc atttgggatc gaaagcagaa aagcatgtcc 601 atgtgtccaa ggaatggatt gcatatttag taacccctaa tggcagtaat aaaactacta 661 ggcaagcttc tctacgaaaa aaaattaggg aacatgatgt ttctaaagcc catggtaaaa 721 ttcaggattt gttaaaggaa tcaactaatg attcaatttg taatttagtg cataaacaaa 781 ataataaaaa tattgatgct actgtaaaag ttttcaatac tgtttacagt ttagtaaaac 841 ataacagacc tttatctgat attgaggggg caagagaatt acaggaaaaa aatggagagg 901 taaattgttt aaatacacgt tacagtgcaa caagaatagc agaacatatt gcaaaagaaa 961 tgaagatgaa gatatttaag aatattatag aagagaatgc caaaatctgt atcataattg 1021 atgaggcatc tacagtttca aagaaaacca ccctagtgat ttatctccag tgcacaattc 1081 agtcagctcc tgcacctgtt atgttatttg tggctttaaa agaattggtg tcaactatag 1141 cagagtgtat tgtcaataca ttattgacta ctttaaatga ttgtggtttt acaaatgaat 1201 atttgaaagc aaatttaatt gcattttgtt ctgatggtgc taatacaatc ctgggaagaa 1261 agtctggagt agctacaaaa ttgttagaaa attttcctga aatcatcatt tggaactgtt 1321 taaatcatcg attacaattg tcacttgatg attctatatc cgaaataaaa caaattaatc 1381 atttaaaaat atttattgat aaaatttatt ctatttatca tcaacctaat aaaaatcaaa 1441 ccaagcttct aggaactgta gctaaagaac ttgaaactga aattattaaa attggtcgag 1501 taatgggacc aagatgggcg gcatgtagtt tacaagctgc tactgctgta tggcatgcat 1561 atcctatatt atatatgcat ttttctcatt cttactctgg tttggcgaag agattagcta 1621 acattaattt cttacaagac cttgctttaa tgattgacat tcttgaagaa ttttcagtac 1681 tttcaactgc attacagtca agatcaacta atattaagaa agcacaaaaa ttgatcaaac 1741 gtaccataag agctttggaa aatttaaaaa ttggtactgg aaagtatgaa tctcaaattg 1801 aagatttgat caagtcagat aagtttaaag atattccatt taataaaaac aataaattta 1861 atgctcttcc taggagtata ttactagaca atataattca gcacatgaac ctacgccttt 1921 tatctgacag aaaccatgaa gatattttta attactttga tttgctggaa ccttccacat 1981 ggCCTTATGA AGAAATAACT TCACCATGGA TAGCTGGTGA AAAAACATTA TTTCATTTGT 2041 GTAAAATTTT AAAATATGAA GTTGATTTGA ATGATTTTCG GGAATTTGTA AATAATAATA 2101 TAAAATCAAA CAATGTTTCA ATTCCTACAA CTATATACAA AGCTAAAAAG ATAGTTAGCA 2161 CCATTGCAAT CAATAGTGCT GAAGCTGAAA GGGGTTTCAA TTTAATGAAC ATAATTTGTA 2221 CAAGGGTGAG AAATAGTTTA ACAATAGATC ATGTATCAGA TTTAATGACA ATAAATTTAC 2281 TGGGGAAAGA ATTAGCAGAT TGGGATGCAA CACCGTTTGT AAAATCTTGG TCAAATTGCA 2341 ACCACAGGTT GGCTACAGAT ACAAGAGTTC GGCAAAAGTC AACAAAAGTC TTCCATGAGA 2401 ATCAATTGGC TATATGGAAC TTAAAATACC CAACTTTCTT GTACAAAGTG GTTGATATCG 2461 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGCCCGT 2521 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCCACTTC 2581 GCTGCCACTT ATGATGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2641 aagatt