Construct: ORF TRCN0000471717
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016719.1_s317c1
- Derived from:
- ccsbBroadEn_05148
- DNA Barcode:
- TACAAAAACCTTAAGCATCTTGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC30A8 (169026)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471717
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 169026 | SLC30A8 | solute carrier family 30 me... | NM_001172811.2 | 100% | 100% | |
| 2 | human | 169026 | SLC30A8 | solute carrier family 30 me... | NM_001172813.2 | 100% | 100% | |
| 3 | human | 169026 | SLC30A8 | solute carrier family 30 me... | NM_001172814.2 | 100% | 100% | |
| 4 | human | 169026 | SLC30A8 | solute carrier family 30 me... | NM_001172815.2 | 100% | 100% | |
| 5 | human | 169026 | SLC30A8 | solute carrier family 30 me... | XM_024447083.1 | 100% | 100% | |
| 6 | human | 169026 | SLC30A8 | solute carrier family 30 me... | NM_173851.3 | 86.7% | 86.7% | 1_147del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1029
- ORF length:
- 960
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtaccactgc cacagtggct ccaagcccac agaaaagggg gcgaatgagt 121 acgcctatgc caagtggaaa ctctgttctg cttcagcaat atgcttcatt ttcatgattg 181 cagaggtcgt gggtgggcac attgctggga gtcttgctgt tgtcacagat gctgcccacc 241 tcttaattga cctgaccagt ttcctgctca gtctcttctc cctgtggttg tcatcgaagc 301 ctccctctaa gcggctgaca tttggatggc accgagcaga gatccttggt gccctgctct 361 ccatcctgtg catctgggtg gtgactggcg tgctagtgta cctggcatgt gagcgcctgc 421 tgtatcctga ttaccagatc caggcgactg tgatgatcat cgtttccagc tgcgcagtgg 481 cggccaacat tgtactaact gtggttttgc accagagatg ccttggccac aatcacaagg 541 aagtacaagc caatgccagc gtcagagctg cttttgtgca tgcccttgga gatctatttc 601 agagtatcag tgtgctaatt agtgcactta ttatcTACTT TAAGCCAGAG TATAAAATAG 661 CCGACCCAAT CTGCACATTC ATCTTTTCCA TCCTGGTCTT GGCCAGCACC ATCACTATCT 721 TAAAGGACTT CTCCATCTTA CTCATGGAAG GTGTGCCAAA GAGCCTGAAT TACAGTGGTG 781 TGAAAGAGCT TATTTTAGCA GTCGACGGGG TGCTGTCTGT GCACAGCCTG CACATCTGGT 841 CTCTAACAAT GAATCAAGTA ATTCTCTCAG CTCATGTTGC TACAGCAGCC AGCCGGGACA 901 GCCAAGTGGT TCGGAGAGAA ATTGCTAAAG CCCTTAGCAA AAGCTTTACG ATGCACTCAC 961 TCACCATTCA GATGGAATCT CCAGTTGACC AGGACCCCGA CTGCCTTTTC TGTGAAGACC 1021 CCTGTGACTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1081 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1141 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATACAAA AACCTTAAGC ATCTTGAAAC 1201 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt