Construct: ORF TRCN0000471733
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003381.1_s317c1
- Derived from:
- ccsbBroadEn_10829
- DNA Barcode:
- ACATAGGACCCATATCGGGTGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADAM2 (2515)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471733
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2515 | ADAM2 | ADAM metallopeptidase domain 2 | XM_011544480.1 | 100% | 100% | |
| 2 | human | 2515 | ADAM2 | ADAM metallopeptidase domain 2 | XM_011544479.1 | 95% | 95% | 1236_1325del |
| 3 | human | 2515 | ADAM2 | ADAM metallopeptidase domain 2 | XM_005273468.1 | 82.1% | 82.1% | 513_890del |
| 4 | human | 2515 | ADAM2 | ADAM metallopeptidase domain 2 | NM_001278113.2 | 80.8% | 80.8% | 513_833del;1557_1646del |
| 5 | human | 2515 | ADAM2 | ADAM metallopeptidase domain 2 | NM_001464.5 | 78.7% | 78.7% | 513_890del;1614_1703del |
| 6 | human | 2515 | ADAM2 | ADAM metallopeptidase domain 2 | NM_001278114.2 | 77.4% | 77.4% | 513_890del;1608_1609ins99 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1803
- ORF length:
- 1737
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gcgcgtcttg tttctgctca gcgggctcgg cgggctgcgg atggacagta 121 attttgatag tttacctgtg caaattacag ttccggagaa aatacggtca ataataaagg 181 aaggaattga atcgcaggca tcctacaaaa ttgtaattga agggaaacca tatactgtga 241 atttaatgca aaaaaacttt ttaccccata attttagagt ttacagttat agtggcacag 301 gaattatgaa accacttgac caagattttc agaatttctg ccactaccaa gggtatattg 361 aaggttatcc aaaatctgtg gtgatggtta gcacatgtac tggactcagg ggcgtactac 421 agtttgaaaa tgttagttat ggaatagaac ccctggagtc ttcagttggc tttgaacatg 481 taatttacca agtaaaacat aagaaagcag atgtttcctt atataatgag aaggatattg 541 aatcaagaga tctgtccttt aaattacaaa gcgtagagca ccccagaacc ataagtctgg 601 aatcacttgc agttatttta gctcaattat tgagccttag tatggggatc acttatgatg 661 acattaacaa atgccagtgc tcaggagctg tctgcattat gaatccagaa gcaattcatt 721 tcagtggtgt gaagatcttt agtaactgca gcttcgaaga ctttgcacat tttatttcaa 781 agcagaagtc ccagtgtctt cacaatcagc ctcgcttaga tccttttttc aaacagcaag 841 cagtgtgtgg taatgcaaag ctggaagcag gagaggagtg tgactgtggg actgaacagg 901 attgtgccct tattggagaa acatgctgtg atattgccac atgtagattt aaagccggtt 961 caaactgtgc tgaaggacca tgctgcgaaa actgtctatt tatgtcaaaa gaaagaatgt 1021 gtaggccttc ctttgaagaa tgcgacctcc ctgaatattg caatggatca tctgcatcat 1081 gcccagaaaa ccactatgtt cagactgggc atccgtgtgg actgaatcaa tggatctgta 1141 tagatggagt ttgtatgagt ggggataaac aatgtacaga cacatttggc aaagaagtag 1201 agtttggccc ttcagaatgt tattctcacc ttaattcaaa gactgatgta tctggaaact 1261 gtggtataag tgattcagga tacacacagt gtgaagctga tggacatctc tgcattgctg 1321 tggaatttgc cagtgatcat gcagacagcc aaaagatgtg gataaaagat ggaacttctt 1381 gtggttcaaa taaggtttgc aggaatcaaa gatgtgtgag TTCTTCATAC TTGGGTTATG 1441 ATTGTACTAC TGACAAATGC AATGATAGAG GTGTATGCAA TAACAAAAAG CACTGTCACT 1501 GTAGTGCTTC ATATTTACCT CCAGATTGCT CAGTTCAATC AGATCTATGG CCTGGTGGGA 1561 GTATTGACAG TGGCAATTTT CCACCTGTAG CTATACCAGC CAGACTCCCT GAAAGGCGCT 1621 ACATTGAGAA CATTTACCAT TCCAAACCAA TGAGATGGCC ATTTTTCTTA TTCATTCCTT 1681 TCTTTATTAT TTTCTGTGTA CTGATTGCTA TAATGGTGAA AGTTAATTTC CAAAGGAAAA 1741 AATGGAGAAC TGAGGACTAT TCAAGCGATG AGCAACCTGA AAGTGAGAGT GAACCTAAAG 1801 GGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1861 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1921 GGCTTTATAT ATCTTGTGGA AAGGACGAAC ATAGGACCCA TATCGGGTGG CGACGCGTTA 1981 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt