Construct: ORF TRCN0000471762
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010326.1_s317c1
- Derived from:
- ccsbBroadEn_04738
- DNA Barcode:
- GACCGCCTGATACTGACCGACGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SFXN4 (119559)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471762
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 119559 | SFXN4 | sideroflexin 4 | NM_213649.2 | 99.9% | 100% | 354A>G |
| 2 | human | 119559 | SFXN4 | sideroflexin 4 | XM_005269525.5 | 97.2% | 97.3% | 252_253ins27;327A>G |
| 3 | human | 119559 | SFXN4 | sideroflexin 4 | XR_945603.3 | 65.4% | (many diffs) | |
| 4 | human | 119559 | SFXN4 | sideroflexin 4 | XM_005269526.2 | 65.4% | 65.5% | 0_1ins348;6A>G |
| 5 | human | 119559 | SFXN4 | sideroflexin 4 | XM_005269527.1 | 65.4% | 65.5% | 0_1ins348;6A>G |
| 6 | human | 119559 | SFXN4 | sideroflexin 4 | XM_011539282.2 | 65.4% | 65.5% | 0_1ins348;6A>G |
| 7 | human | 119559 | SFXN4 | sideroflexin 4 | XM_024447793.1 | 65.4% | 65.5% | 0_1ins348;6A>G |
| 8 | human | 119559 | SFXN4 | sideroflexin 4 | NR_110305.1 | 64.6% | (many diffs) | |
| 9 | human | 119559 | SFXN4 | sideroflexin 4 | XR_001747023.1 | 60.3% | (many diffs) | |
| 10 | human | 119559 | SFXN4 | sideroflexin 4 | XR_001747022.1 | 56.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1077
- ORF length:
- 1011
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cctggaacag gaggaggaaa cgcaacctgg gcggctccta ggacgcagag 121 acgccgtccc cgccttcatt gagcccaacg tgcgcttctg gatcaccgag cgccaatcct 181 ttattcgacg atttcttcaa tggacagaat tattagatcc tacaaatgtg ttcatttcag 241 ttgaaagtat agaaaactcg aggcaactat tgtgcacaaa tgaagatgtt tccagccctg 301 cctcggcgga ccaaaggata caagaagctt ggaagcggag tcttgcaaca gtgcatcccg 361 acagcagcaa cctgatcccc aagctttttc gacctgcagc gttcctgcct ttcatggcgc 421 ccacggtatt tttgtcaatg acgccactga aagggatcaa gtccgtgatt ttacctcagg 481 ttttcctctg tgcctacatg gcagcgttca acagcatcaa tggaaacaga agttacactt 541 gtaagccact agaaagatca ttactaatgg cgggagccgt tgcttcttca actttcttag 601 gagtaatccc TCAGTTTGTC CAGATGAAGT ATGGCCTGAC TGGCCCTTGG ATTAAAAGAC 661 TCTTACCTGT GATCTTCCTC GTGCAAGCCA GTGGAATGAA TGTCTACATG TCCCGAAGTC 721 TTGAATCCAT TAAGGGGATT GCGGTCATGG ACAAGGAAGG CAATGTCCTG GGTCATTCCA 781 GAATTGCTGG GACAAAGGCT GTTAGAGAAA CGCTAGCATC CAGAATAGTG CTGTTTGGGA 841 CCTCAGCTCT GATTCCTGAA GTCTTCACCT ACTTTTTTAA AAGGACCCAG TATTTCAGGA 901 AAAACCCAGG GTCATTGTGG ATTTTGAAAC TGTCTTGTAC TGTCCTGGCA ATGGGACTGA 961 TGGTGCCATT TTCTTTTAGT ATATTTCCAC AGATTGGACA GATACAGTAC TGTAGTCTTG 1021 AAGAGAAAAT TCAGTCTCCA ACAGAAGAAA CAGAAATCTT TTATCACAGA GGGGTGTACC 1081 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1141 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1201 ATATATCTTG TGGAAAGGAC GAGACCGCCT GATACTGACC GACGACACGC GTTAAGTCga 1261 caatcaacct ctggattaca aaatttgtga aagatt