Construct: ORF TRCN0000471777
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017936.1_s317c1
- Derived from:
- ccsbBroadEn_03533
- DNA Barcode:
- CCTACGGCCGGACGCCGAGAATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDCA8 (55143)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471777
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55143 | CDCA8 | cell division cycle associa... | NM_001256875.2 | 100% | 100% | |
| 2 | human | 55143 | CDCA8 | cell division cycle associa... | NM_018101.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 906
- ORF length:
- 840
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tcctaggaag ggcagtagtc gggtggccaa gaccaactcc ttacggaggc 121 ggaagctcgc ctcctttctg aaagacttcg accgtgaagt ggaaatacga atcaagcaaa 181 ttgagtcaga caggcagaac ctcctcaagg aggtggataa cctctacaac atcgagatcc 241 tgcggctccc caaggctctg cgcgagatga actggcttga ctacttcgcc cttggaggaa 301 acaaacaggc cctggaagag gcggcaacag ctgacctgga tatcaccgaa ataaacaaac 361 taacagcaga agctattcag acacccctga aatctgccaa aacacgaaag gtaatacagg 421 tagatgaaat gatagtggaa gaggaagaag aagaagaaaa tgaacgtaag aatcttcaaa 481 ctgcaagagt caaaaggtgt cctccatcca agaagagaac tcagtccata caaggaaaag 541 gaaaagggaa aaggtcaagc cgtgctaaca ctgttacccc agccgtgggc cgattGGAGG 601 TGTCCATGGT CAAACCAACT CCAGGCCTGA CACCCAGGTT TGACTCAAGG GTCTTCAAGA 661 CCCCTGGCCT GCGTACTCCA GCAGCAGGAG AGCGGATTTA CAACATCTCA GGGAATGGCA 721 GCCCTCTTGC TGACAGCAAA GAGATCTTCC TCACTGTGCC AGTGGGCGGC GGAGAGAGCC 781 TGCGATTATT GGCCAGTGAC TTGCAGAGGC ACAGTATTGC CCAGCTGGAT CCAGAGGCCT 841 TGGGAAACAT TAAGAAGCTC TCCAACCGTC TCGCCCAAAT CTGCAGCAGC ATACGGACCC 901 ACAAATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 961 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1021 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCTACGGCC GGACGCCGAG AATGTACGCG 1081 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt