Transcript: Human NM_001256875.2

Homo sapiens cell division cycle associated 8 (CDCA8), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-05-21
Taxon:
Homo sapiens (human)
Gene:
CDCA8 (55143)
Length:
2371
CDS:
176..1018

Additional Resources:

NCBI RefSeq record:
NM_001256875.2
NBCI Gene record:
CDCA8 (55143)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001256875.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000298519 CGGAGAGAGCCTGCGATTATT pLKO_005 880 CDS 100% 15.000 21.000 N CDCA8 n/a
2 TRCN0000007897 CGAAAGGTAATACAGGTAGAT pLKO.1 515 CDS 100% 4.950 6.930 N CDCA8 n/a
3 TRCN0000007898 CCTGGATATCACCGAAATAAA pLKO.1 445 CDS 100% 15.000 10.500 N CDCA8 n/a
4 TRCN0000293523 CCTGGATATCACCGAAATAAA pLKO_005 445 CDS 100% 15.000 10.500 N CDCA8 n/a
5 TRCN0000293524 AGCAGCAGGAGAGCGGATTTA pLKO_005 790 CDS 100% 13.200 9.240 N CDCA8 n/a
6 TRCN0000007896 CCCTGGAGAATCATGTGCTAT pLKO.1 2076 3UTR 100% 4.950 3.465 N CDCA8 n/a
7 TRCN0000007899 CGCCTCCTTTCTGAAAGACTT pLKO.1 238 CDS 100% 4.950 3.465 N CDCA8 n/a
8 TRCN0000007900 GTTTGACTCAAGGGTCTTCAA pLKO.1 748 CDS 100% 4.950 3.465 N CDCA8 n/a
9 TRCN0000293451 GTTTGACTCAAGGGTCTTCAA pLKO_005 748 CDS 100% 4.950 3.465 N CDCA8 n/a
10 TRCN0000293525 TGCTTGAGACCGGGAGGTTTA pLKO_005 1443 3UTR 100% 10.800 6.480 N CDCA8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001256875.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03533 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_03533 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000471777 CCTACGGCCGGACGCCGAGAATGT pLX_317 37.3% 100% 100% V5 n/a
4 ccsbBroadEn_08476 pDONR223 100% 99.8% 99.6% None 36G>T n/a
5 ccsbBroad304_08476 pLX_304 0% 99.8% 99.6% V5 36G>T n/a
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