Construct: ORF TRCN0000471785
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001864.1_s317c1
- Derived from:
- ccsbBroadEn_05402
- DNA Barcode:
- AGCACCTACCCTTCCGCCGAAGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIAA0825 (285600)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471785
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285600 | KIAA0825 | KIAA0825 | NM_173665.3 | 100% | 100% | |
| 2 | human | 285600 | KIAA0825 | KIAA0825 | XM_017009376.2 | 100% | 100% | |
| 3 | human | 285600 | KIAA0825 | KIAA0825 | XM_017009377.1 | 100% | 100% | |
| 4 | human | 285600 | KIAA0825 | KIAA0825 | XM_017009374.1 | 25.8% | 25.8% | 971T>G;973_3753del |
| 5 | human | 285600 | KIAA0825 | KIAA0825 | XM_011543333.2 | 25.8% | 25.7% | 971T>G;973_3756del |
| 6 | human | 285600 | KIAA0825 | KIAA0825 | XM_011543334.2 | 25.8% | 25.7% | 971T>G;973_3756del |
| 7 | human | 285600 | KIAA0825 | KIAA0825 | XM_017009373.1 | 25.8% | 25.7% | 971T>G;973_3756del |
| 8 | human | 285600 | KIAA0825 | KIAA0825 | XM_011543332.1 | 25.6% | 25.5% | 971T>G;973_3789del |
| 9 | human | 285600 | KIAA0825 | KIAA0825 | NM_001145678.2 | 25.3% | 25.3% | 971T>G;973_3825del |
| 10 | human | 285600 | KIAA0825 | KIAA0825 | XM_006714588.2 | 25.2% | 25.2% | 971T>G;973_3840del |
| 11 | human | 285600 | KIAA0825 | KIAA0825 | XM_011543331.1 | 25.2% | 25.2% | 971T>G;973_3840del |
| 12 | human | 285600 | KIAA0825 | KIAA0825 | XM_011543330.2 | 25.2% | 25.1% | 971T>G;973_3849del |
| 13 | human | 285600 | KIAA0825 | KIAA0825 | XM_011543324.2 | 24.1% | 24% | 971T>G;973_4023del |
| 14 | human | 285600 | KIAA0825 | KIAA0825 | XM_011543325.3 | 24.1% | 24% | 971T>G;973_4023del |
| 15 | human | 285600 | KIAA0825 | KIAA0825 | XM_011543327.2 | 24.1% | 24% | 971T>G;973_4023del |
| 16 | human | 285600 | KIAA0825 | KIAA0825 | XM_011543328.3 | 24.1% | 24% | 971T>G;973_4023del |
| 17 | human | 285600 | KIAA0825 | KIAA0825 | XM_011543329.2 | 24.1% | 24% | 971T>G;973_4023del |
| 18 | human | 285600 | KIAA0825 | KIAA0825 | XM_011543335.2 | 15.7% | 15.7% | 0_1ins336;635T>G;637_3687del |
| 19 | human | 285600 | KIAA0825 | KIAA0825 | XR_001742053.2 | 15.4% | 1_257del;1228T>G;1230_6282del | |
| 20 | human | 285600 | KIAA0825 | KIAA0825 | XR_948246.2 | 8.9% | 1_257del;1228T>G;1230_10891del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1038
- ORF length:
- 972
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ttgggatgat gaatattctc ataattcttt tgacctacat tgtttgttaa 121 actcatttcc tggagacttg gagtttgagc agatcttcag tgacattgat gaaaagattg 181 aacaaaatgc tgcaagcata aaacattgca ttaaagagat acagtccgaa attaacaaac 241 aatgtccagg tgtgcagctg caaacaacaa ctgactgctt tgaatggcta actaactata 301 attacagtac atctgaatca tctttcattt ctcatggaga cttgataaaa tttttcaaaa 361 cactgcaaga tttgttgaag aatgaacaaa atcaagaaga aatgacattg gatttacttt 421 gggacctctc ctgccacagc agcgtttcat tcccatcaac cctaagtgga acatctttcc 481 atttcctctc taggacgtct cttcattctg ttgaagataa ttcctctatg gatgtcaagt 541 ctatgtGGGA TGATATAAGA CTGCATCTTC GACGCTTCTT AGTGAGCAAA TTACAAAGCC 601 ATAATGAAAT AAACAATTCA CAGCAAAAAA TTTTATTGAA AAAGCAGTGC TTACAACAAC 661 TCTTGTTTCT TTATCCAGAA TCAGAAGTTA TAATCAAATA CCAAAACATA CAGAATAAAC 721 TGTTGGCTAA TCTTCTGTGG AACTGCTTTC CTTCTTACAA CAGAGATTCA AATTTAGATG 781 TAATAGCTCA TGGATATCAA AGTACAATGC TTAAGTTATA CTCAGTAATA AAAGAGGATT 841 TTAACACACT ATGTGAAATT TTAGCTCCAT CTTCAATGGT GAAATTCATT AAAGAAACTT 901 ACCTGGATAC TGTTACAGAA GAAATGGCAA AATTTCTTGA AAATTTTTGT GAGCTGCAGT 961 TCAGAGAAAA TGCTGTTCGT GTGGTTAAAA CAAGCAAGAG CTCCAGCAAG CATAGAGGAG 1021 CAGTGCATGC TTTGGGTTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1081 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1141 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAGCACCT ACCCTTCCGC 1201 CGAAGATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt