Construct: ORF TRCN0000471822
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016594.1_s317c1
- Derived from:
- ccsbBroadEn_00499
- DNA Barcode:
- TACCAACAAAGCGACGGGTCCGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ELK3 (2004)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471822
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2004 | ELK3 | ETS transcription factor ELK3 | NM_005230.4 | 100% | 100% | |
| 2 | human | 2004 | ELK3 | ETS transcription factor ELK3 | XM_006719275.3 | 100% | 100% | |
| 3 | human | 2004 | ELK3 | ETS transcription factor ELK3 | NM_001303511.2 | 19.9% | 18.6% | (many diffs) |
| 4 | mouse | 13713 | Elk3 | ELK3, member of ETS oncogen... | NM_013508.2 | 89.1% | 91.9% | (many diffs) |
| 5 | mouse | 13713 | Elk3 | ELK3, member of ETS oncogen... | NM_001322268.1 | 54.7% | 44.8% | (many diffs) |
| 6 | mouse | 13713 | Elk3 | ELK3, member of ETS oncogen... | NM_001322265.1 | 45.1% | 43.4% | (many diffs) |
| 7 | mouse | 13713 | Elk3 | ELK3, member of ETS oncogen... | NM_001282967.1 | 30.7% | 32.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1287
- ORF length:
- 1221
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gagtgcaatc acgctgtggc agttcctgtt gcagttgctg ctggatcaga 121 aacatgagca tttgatctgc tggacctcga acgatggtga attcaagctc ctcaaagcag 181 aagaagtggc caagctgtgg ggactccgaa aaaacaaaac aaatatgaac tatgataagc 241 tgagcagagc cctgcgatac tattatgaca agaacatcat caagaaggtg atcgggcaga 301 agtttgtgta caagtttgtc tctttcccgg agatcctgaa gatggatcct cacgcggtgg 361 agatcagccg ggagagcctt ctgctgcagg acagcgactg caaggcgtct ccggagggcc 421 gcgaggccca caaacacggc ctggccgccc tcagaagcac gagccgcaac gaatacatcc 481 actcaggcct gtactcgtcc ttcaccatta attccctgca gaacccacca gacgccttca 541 aggccatcaa gacggagaag ctggaggagc cgcccgaaga cagccccccc gtggaagaag 601 tcaggactgt gatcaggttt gtgaccaata aaaccgacaa gcacgtcacc aggccggtgg 661 tgtccctgcc ttccacgtca gaggctgcgg cggcgtccgc cttcctggcc tcgtccgtct 721 cggccaagat ctcctcttta atgttgccaa acgctgccag tatttcatcc gcctcaccct 781 tctcatctcg gtccccgtcc ctgtccccca actcacccct cccttctgaa cacagaagcc 841 tcttcctgga ggccgcctgc catgacTCCG ATTCCCTGGA GCCCTTGAAC CTGTCATCGG 901 GCTCCAAGAC CAAGTCTCCA TCTCTTCCCC CAAAGGCCAA AAAACCCAAA GGCTTGGAAA 961 TCTCAGCGCC CCCGCTGGTG CTCTCCGGCA CCGACATCGG CTCCATCGCC CTCAACAGCC 1021 CAGCCCTCCC CTCGGGATCC CTCACCCCAG CCTTCTTCAC CGCACAGACA CCAAATGGAT 1081 TGCTTCTGAC TCCGAGTCCA CTGCTCTCCA GCATACATTT CTGGAGCAGC CTTAGTCCAG 1141 TTGCTCCGCT GAGTCCTGCC AGGCTGCAAG GGCCAAGCAC GCTGTTCCAG TTCCCCACAC 1201 TGCTTAATGG CCACATGCCA GTGCCAATCC CCAGTCTGGA CAGAGCTGCT TCTCCAGTAC 1261 TGCTTTCTTC AAACTCTCAG AAATCCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1321 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1381 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATACCAACA 1441 AAGCGACGGG TCCGATACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1501 aagatt