Construct: ORF TRCN0000471829
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010868.1_s317c1
- Derived from:
- ccsbBroadEn_04513
- DNA Barcode:
- AGGCCTGTCACTCTTGATGCCAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCAF15 (90379)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471829
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90379 | DCAF15 | DDB1 and CUL4 associated fa... | NM_138353.3 | 100% | 100% | |
| 2 | mouse | 212123 | Dcaf15 | DDB1 and CUL4 associated fa... | XM_006530808.1 | 86.1% | 90.8% | (many diffs) |
| 3 | mouse | 212123 | Dcaf15 | DDB1 and CUL4 associated fa... | NM_172502.3 | 81% | 85.4% | (many diffs) |
| 4 | mouse | 212123 | Dcaf15 | DDB1 and CUL4 associated fa... | XM_011248336.1 | 58% | 60.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1869
- ORF length:
- 1800
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgcccagc tcgaaatcgg agcggaacag cggggctggg agcggcggcg 121 gcggccccgg gggagccgga gggaagcggg cagcagggcg gcggcgggag cacgtcctca 181 agcagctgga gcgggtcaag atcagcggac agctctcccc tcgcctcttc cggaagctgc 241 ctccccgggt gtgcgtgtcc ctcaagaaca ttgtggatga ggacttcctc tatgcaggac 301 atatcttcct gggcttttcc aaatgcggcc gctacgtcct ctcctacacc agcagcagtg 361 gggatgacga cttctccttc tacatctacc atctgtactg gtgggagttc aacgttcaca 421 gcaagctcaa gctggtccgg caggttcggc tattccagga cgaggagatc tacagcgacc 481 tgtacctgac cgtatgcgag tggcccagcg acgcctccaa ggtcatcgtc ttcggcttca 541 acacccgctc ggccaacggg atgctcatga acatgatgat gatgagtgac gagaaccacc 601 gtgacatcta cgtcagcacc gtggccgtgc caccgccagg ccgctgtgct gcttgccagg 661 atgccagccg agcccaccca ggagacccga atgcacagtg cctacggcat ggcttcatgc 721 tgcacaccaa gtaccaggtg gtctacccct tccccacctt ccagcccgcc ttccagctca 781 agaaggacca ggtggtgctg ctcaacacca gctactccct ggtggcctgc gccgtctccg 841 tccactcggc aggtgacagg agtttctgcc aaatcctgta tgaccacagc acctgccccc 901 tggcgcctgc cagcccccct gagccccaga gcccagagct gccccctgcc ctccccagct 961 tctgccctga ggcggcccca gcccgttctt ctgggtctcc tgagccctcg cccgccattg 1021 ccaaagccaa ggagtttgtg gctgacatct tccgccgggc caaagaggcc aagggcgggg 1081 tccctgagga agcccggcct gccctgtgcc caggaccctc tggcagccgc tgccgtgcgc 1141 actctgagcc cctagccctg tgtggagaga cggcaccccg ggacagcccc cctgcctcgg 1201 aggcacctgc ctccgagcct ggctatgtca actacaccaa gctgtactat gtgctggagt 1261 ccggagaggg gacggagccg gaggatgagt tggaggacga caagatctcc ctgcccttcg 1321 tggtgactga tcttcgtggc cgcaacctgc ggcccatgcg ggagcggact gctgtccagg 1381 gccagtacct gacagtggag cagctcacac tagacttcga atatgttatc aatgaggtca 1441 tccgccacga cgctaccTGG GGCCATCAGT TCTGTTCTTT CAGCGACTAT GACATCGTCA 1501 TTCTGGAGGT CTGCCCAGAA ACCAACCAGG TCCTCATCAA CATTGGCCTG CTGCTCCTGG 1561 CCTTCCCGTC CCCCACTGAG GAGGGCCAGC TCCGACCAAA GACCTATCAC ACCAGCCTCA 1621 AGGTGGCATG GGACCTCAAC ACAGGGATCT TCGAGACAGT CAGTGTAGGC GACCTGACTG 1681 AGGTCAAAGG GCAGACCAGC GGCAGTGTCT GGAGCTCCTA CCGCAAGAGC TGCGTGGACA 1741 TGGTCATGAA GTGGCTGGTG CCGGAGAGCA GCGGCCGCTA CGTCAACAGG ATGACCAATG 1801 AGGCGCTGCA CAAAGGGTGC TCCCTGAAGG TTCTGGCGGA CAGCGAGCGA TATACGTGGA 1861 TCGTGCTGTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1921 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1981 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGGCCT GTCACTCTTG ATGCCAAGAC 2041 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt