Construct: ORF TRCN0000471840
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015879.1_s317c1
- Derived from:
- ccsbBroadEn_11142
- DNA Barcode:
- CCTATACTTTAACCATTGAGGGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC22A2 (6582)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471840
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6582 | SLC22A2 | solute carrier family 22 me... | NM_003058.4 | 42.3% | 39.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 792
- ORF length:
- 726
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc caccaccgtg gacgatgtcc tggagcatgg aggggagttt cactttttcc 121 agaagcaaat gtttttcctc ttggctctgc tctcggctac cttcgcgccc atctacgtgg 181 gcatcgtctt cctgggcttc acccctgacc accgctgccg gagccccgga gtggccgagc 241 tgagtctgcg ctgcggctgg agtcctgcag aggaactgaa ctacacggtg ccgggcccag 301 gacctgcggg cgaagcctcc ccaagacagt gtaggcgcta cgaggtggac tggaaccaga 361 gcaCCTTCGA CTGCGTGGAC CCCCTGGCCA GCCTGGACAC CAACAGGAGC CGCCTGCCAC 421 TGGGCCCCTG CCGGGACGGC TGGGTGTACG AGACGCCTGG CTCGTCCATC GTCACCGAGT 481 TTAACCTGGT ATGTGCCAAC TCCTGGATGT TGGACCTATT CCAGTCATCA GTGAATGTAG 541 GATTCTTTAT TGGCTCTATG AGTATCGGCT ACATAGCAGA CAGGTTTGGC CGTAAGCTCT 601 GCCTCCTAAC TACAGTCCTC ATAAATGCTG CAGCTGGAGT TCTCATGGCC ATTTCCCCAA 661 CCTATACGTG GATGTTAATT TTTCGCTTAA TCCAAGGACT GGTCAGCAAA GCAGGCTGGT 721 TAATAGGCTA CATCCTGAGT AAGAATGTTT GTGCTTGCAA CTGTGAGAAC AAAGCAACCT 781 CGCTGCCAAA ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 841 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 901 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCT ATACTTTAAC CATTGAGGGA 961 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t